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SCRIB

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GENERAL INFORMATION

Gene name

 SCRIB

Gene description

 Scribbled planar cell polarity protein

Protein class

 Disease related genes
Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 5
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HUMAN PROTEIN ATLAS INFORMATION

Summary

 Mainly localized to the plasma membrane & cell junctions. In addition localized to the nucleoplasm & rods & rings.

RNA cell category

 Expressed in all

Protein evidence

 Evidence at protein level

Main location

 Localized to the Plasma membrane (validated), Cell Junctions (validated)

Additional location

 In addition localized to the Nucleoplasm (validated), Rods & Rings (approved)

siRNA

 The location(s) are Validated based on siRNA mediated gene silencing.

DATA RELIABILITY

Reliability score

 Validated based on 2 antibodies.
HPA023557 , HPA064312
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ASSAYS
RNA expression
Human cells
Custom data
siRNA

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all
Organ
Origin
Category
Expression
Alphabetical
Cell lines sorted after organ of phenotypic resemblance.

HUMAN CELLS

Summary

 Mainly localized to the plasma membrane & cell junctions. In addition localized to the nucleoplasm & rods & rings.

Main location

 Plasma membrane (validated), Cell Junctions (validated)

Additional location

 Nucleoplasm (validated), Rods & Rings (approved)

 

 

 
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Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

Location

Single-cell variation

Cell cycle dependent variation
HPA023557 A-431 Nucleoplasm
Plasma membrane
HPA023557 U-2 OS Nucleoplasm
Plasma membrane
Cell Junctions
HPA023557 U-251 MG Nucleoplasm
Plasma membrane
HPA064312 HEK 293 Plasma membrane
Cell Junctions
HPA064312 MCF7 Nucleoplasm
Plasma membrane
Cell Junctions
HPA064312 U-2 OS Plasma membrane
Cell Junctions
Rods & Rings

CUSTOM DATA

 

 

 
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Thumbnail

Antibody

Cell line

Assay

Description
HPA064312 U-2 OS Image U-2 OS cells were treated with Ribavirin to induce the formation of Rods & Rings. Co-localization shows that SCRIB localizes to Rods & Rings as the staining overlaps with a marker for Rods & Rings.

siRNA VALIDATION
 

Relative fluorescence intensity (RFI)

Antibody

 HPA023557

siRNA validation

 Validated: Significant downregulation > 25% by both siRNA:s.

Analysis performed in

 Cytoplasmic region of segmented cells in 10x-images

siRNA 1

siRNA 2

RFI

 61%61%

p-value

 < 0.01< 0.01
 

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

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Thumbnail 40x

Antibody

Sample

Cell line
HPA023557 siRNA 1 U-2 OS
HPA023557 siRNA 2 U-2 OS
HPA023557 scrambled U-2 OS

GENE INFORMATION

Gene name

 SCRIB (HGNC Symbol)

Synonyms

 KIAA0147, SCRB1, Vartul

Description

 Scribbled planar cell polarity protein (HGNC Symbol)

Entrez gene summary

 This gene encodes a protein that was identified as being similar to the Drosophila scribble protein. The mammalian protein is involved in tumor suppression pathways. As a scaffold protein involved in cell polarization processes, this protein binds to many other proteins. The encoded protein binds to papillomavirus E6 protein via its PDZ domain and the C-terminus of E6. Two alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Chromosome

 8

Cytoband

 q24.3

Chromosome location (bp)

 143790920 - 143815379

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000180900 (version 83.38)

Entrez gene

 23513

UniProt

 Q14160 (UniProt - Evidence at protein level)

neXtProt

 NX_Q14160

Antibodypedia

 SCRIB antibodies

PROTEIN BROWSER
SCRIB-001
SCRIB-002
SCRIB-003
SCRIB-004
SCRIB-007

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
SCRIB-001
ENSP00000322938
ENST00000320476
Q14160 [Direct mapping]
Protein scribble homolog
Show all
Predicted intracellular proteins
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001843 [neural tube closure]
GO:0001921 [positive regulation of receptor recycling]
GO:0005515 [protein binding]
GO:0005654 [nucleoplasm]
GO:0005886 [plasma membrane]
GO:0005911 [cell-cell junction]
GO:0005913 [cell-cell adherens junction]
GO:0008283 [cell proliferation]
GO:0016032 [viral process]
GO:0016337 [single organismal cell-cell adhesion]
GO:0016477 [cell migration]
GO:0030027 [lamellipodium]
GO:0031252 [cell leading edge]
GO:0034750 [Scrib-APC-beta-catenin complex]
GO:0035089 [establishment of apical/basal cell polarity]
GO:0042734 [presynaptic membrane]
GO:0043065 [positive regulation of apoptotic process]
GO:0045211 [postsynaptic membrane]
GO:0045930 [negative regulation of mitotic cell cycle]
GO:0050918 [positive chemotaxis]
GO:0060561 [apoptotic process involved in morphogenesis]
GO:0060603 [mammary gland duct morphogenesis]
GO:0070062 [extracellular exosome]
GO:0071896 [protein localization to adherens junction]
GO:0090630 [activation of GTPase activity]
Show all
 1630 aa
174.9 kDa 		No 0
SCRIB-002
ENSP00000349486
ENST00000356994
Q14160 [Direct mapping]
Protein scribble homolog
Show all
   SCAMPI predicted membrane proteins
Predicted intracellular proteins
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001843 [neural tube closure]
GO:0001921 [positive regulation of receptor recycling]
GO:0002093 [auditory receptor cell morphogenesis]
GO:0005515 [protein binding]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005886 [plasma membrane]
GO:0005911 [cell-cell junction]
GO:0005913 [cell-cell adherens junction]
GO:0008105 [asymmetric protein localization]
GO:0008283 [cell proliferation]
GO:0016032 [viral process]
GO:0016080 [synaptic vesicle targeting]
GO:0016323 [basolateral plasma membrane]
GO:0016331 [morphogenesis of embryonic epithelium]
GO:0016337 [single organismal cell-cell adhesion]
GO:0016477 [cell migration]
GO:0021747 [cochlear nucleus development]
GO:0030027 [lamellipodium]
GO:0031252 [cell leading edge]
GO:0034750 [Scrib-APC-beta-catenin complex]
GO:0035089 [establishment of apical/basal cell polarity]
GO:0035748 [myelin sheath abaxonal region]
GO:0042060 [wound healing]
GO:0042734 [presynaptic membrane]
GO:0043065 [positive regulation of apoptotic process]
GO:0043615 [astrocyte cell migration]
GO:0045211 [postsynaptic membrane]
GO:0045930 [negative regulation of mitotic cell cycle]
GO:0048488 [synaptic vesicle endocytosis]
GO:0050918 [positive chemotaxis]
GO:0060088 [auditory receptor cell stereocilium organization]
GO:0060561 [apoptotic process involved in morphogenesis]
GO:0060603 [mammary gland duct morphogenesis]
GO:0070062 [extracellular exosome]
GO:0071896 [protein localization to adherens junction]
GO:0090630 [activation of GTPase activity]
Show all
 1655 aa
177.7 kDa 		No 0
SCRIB-003
ENSP00000366756
ENST00000377533
Q14160 [Direct mapping]
Protein scribble homolog
Show all
Predicted intracellular proteins
Disease related genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001843 [neural tube closure]
GO:0001921 [positive regulation of receptor recycling]
GO:0005515 [protein binding]
GO:0005654 [nucleoplasm]
GO:0005886 [plasma membrane]
GO:0005911 [cell-cell junction]
GO:0005913 [cell-cell adherens junction]
GO:0008283 [cell proliferation]
GO:0016032 [viral process]
GO:0016337 [single organismal cell-cell adhesion]
GO:0016477 [cell migration]
GO:0030027 [lamellipodium]
GO:0031252 [cell leading edge]
GO:0034750 [Scrib-APC-beta-catenin complex]
GO:0035089 [establishment of apical/basal cell polarity]
GO:0042734 [presynaptic membrane]
GO:0043065 [positive regulation of apoptotic process]
GO:0045211 [postsynaptic membrane]
GO:0045930 [negative regulation of mitotic cell cycle]
GO:0050918 [positive chemotaxis]
GO:0060561 [apoptotic process involved in morphogenesis]
GO:0060603 [mammary gland duct morphogenesis]
GO:0070062 [extracellular exosome]
GO:0071896 [protein localization to adherens junction]
GO:0090630 [activation of GTPase activity]
Show all
 1549 aa
165.3 kDa 		No 0
SCRIB-004
ENSP00000431519
ENST00000526832
H0YCG0 [Direct mapping]
Protein scribble homolog
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005515 [protein binding]
Show all
 650 aa
68.3 kDa 		No 0
SCRIB-007
ENSP00000433546
ENST00000531942
H0YDF9 [Direct mapping]
Protein scribble homolog
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005515 [protein binding]
Show all
 140 aa
14.9 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.