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DHTKD1

ANTIBODY INFORMATION
 
 

Antibody HPA037949

  

Antibody HPA037950

  

Antibody HPA066098

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA037949   HPA037950   HPA066098  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 13   7   16  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA037949

  

Antibody HPA037950

  

Antibody HPA066098

  
Immunocytochemistry

Supported      
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA037949

  

Antibody HPA037950

  

Antibody HPA066098

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.      

Antibody dilution

 1:98      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037949

  

Antibody HPA037950

  

Antibody HPA066098

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 59 cell types		   RNA: detected in 37 tissues
Protein: detected in 59 cell types		   RNA: detected in 37 tissues
Protein: detected in 55 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:175   1:35   1:200  

Literature conformity

 Consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA037949

  

Antibody HPA037950

  

Antibody HPA066098

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 103.1, 34.3   103.1, 23.7   103.1, 34.3  

Antibody dilution

 1:250   1:250   1:320  

PROTEIN ARRAY
 
 

Antibody HPA037949

  

Antibody HPA037950

  

Antibody HPA066098

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:3850  

ANTIGEN INFORMATION
 
 

Antibody HPA037949

  

Antibody HPA037950

  

Antibody HPA066098

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 90   88   76  

Antigen sequence

 
ALPLFWRGYQTERGVYGYRPRKPESREPQGALERPPVDHGLARLVTVYCE
HGHKAAKINPLFTGQALLENVPEIQALVQTLQGPFHTAGL
  
EIKSSYYAKLNDHLNNMAHYRPPALNLQAHWQGLAQPEAQITTWSTGVPL
DLLRFVGMKSVEVPRELQMHSHLLKTHVQSRMEKMMDG
  
LNMGKEEASLEEVLVYLNQIYCGQISIETSQLQSQDEKDWFAKRFEELQK
ETFTTEERKHLSKLMLESQEFDHFLA
 

Matching transcripts

 DHTKD1-001 - ENSP00000263035 [100%]
DHTKD1-006 - ENSP00000388163 [100%]
  DHTKD1-001 - ENSP00000263035 [100%]
DHTKD1-003 - ENSP00000398482 [100%]
  DHTKD1-001 - ENSP00000263035 [100%]
DHTKD1-006 - ENSP00000388163 [91%]
 

Other gene match

      
ANTIGEN VIEW
DHTKD1-001
DHTKD1-003
DHTKD1-006
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.