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Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular medulla cells.
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in medullary cells.
Expression
RNA: detected in 26 tissues Protein: detected in 5 cell types
RNA: detected in 26 tissues Protein: detected in 3 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1500
1:300
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA013138
Antibody CAB016390
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401880)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
30.8
30.8, 11.8, 10.3, 10, 8.8
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA013138
Antibody CAB016390
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
