NPLOC4 - Antibodies - The Human Protein Atlas

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NPLOC4

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA021560

Antibody HPA023295

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA021560

HPA023295

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA021560

Antibody HPA023295

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA021560

Antibody HPA023295

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.

Antibody dilution

1:56

1:75

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA021560

Antibody HPA023295

Standard validation

Supported

Figure description

Immunohistochemical staining of human esophagus shows moderate cytoplasmic and nuclear positivity in squamous epithelial cells.

Immunohistochemical staining of human gallbladder shows moderate cytoplasmic and nuclear positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 66 cell types

RNA: detected in 37 tissues
Protein: detected in 67 cell types

Retrieval

HIER pH6

Antibody dilution

1:40

1:750

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA021560

Antibody HPA023295

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

69.5, 68.1

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA021560

Antibody HPA023295

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA021560

Antibody HPA023295

Antigen

Recombinant protein fragment

Length (aa)

123

109

Antigen sequence

VRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDVDKFGNEITQLARPLP
VEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGETQDFHSLATYLSQ
NTSSVFLDTISDFHLLLFLVTNE

LEPFDEDYLNHLEPPVKHMSFHAYIRKLTGGADKGKFVALENISCKIKSG
CEGHLPWPNGICTKCQPSAITLNRQKYRHVDNIMFENHTVADRFLDFWRK
TGNQHFGYL

Matching transcripts

NPLOC4-001 - ENSP00000331487 [100%]
NPLOC4-002 - ENSP00000363879 [100%]

Other gene match

ANTIGEN VIEW

NPLOC4-001	NPLOC4-002

RELEVANT PUBLICATIONS

Antibody HPA021560

Antibody HPA023295

DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage
Davis EJ et al
Nat Struct Mol Biol 2012;19(11):1093-100

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.