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CAPN12

ANTIBODY INFORMATION
 
 

Antibody HPA043492

  

Antibody HPA053322

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA043492   HPA053322  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA043492

  

Antibody HPA053322

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA043492

  

Antibody HPA053322

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & focal adhesion sites.  

Antibody dilution

   1:21  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043492

  

Antibody HPA053322

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human liver shows strong positivity in hepatocytes.   Immunohistochemical staining of human lateral ventricle shows moderate cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 17 tissues
Protein: detected in 50 cell types		   RNA: detected in 17 tissues
Protein: detected in 29 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:65   1:150  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA043492

  

Antibody HPA053322

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 81, 64.5   81, 64.5  

Antibody dilution

 1:250   1:100  

PROTEIN ARRAY
 
 

Antibody HPA043492

  

Antibody HPA053322

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:850  

ANTIGEN INFORMATION
 
 

Antibody HPA043492

  

Antibody HPA053322

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 63   87  

Antigen sequence

 
DGEFWMELRDFLLHFDTVQICSLSPEVLGPSPEGGGWHVHTFQGRWVRGF
NSGGSQPNAETFW
  
GDEADFTLRVFSERRHTAVEIDDVISADLQSLQGPYLPLELGLEQLFQEL
AGEEEELNASQLQALLSIALEPARAHTSTPREIGLRT
 

Matching transcripts

 CAPN12-001 - ENSP00000331636 [100%]
CAPN12-002 - ENSP00000473156 [100%]
  CAPN12-001 - ENSP00000331636 [100%]
CAPN12-002 - ENSP00000473156 [100%]
 

Other gene match

    
ANTIGEN VIEW
CAPN12-001
CAPN12-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.