EXOC7 - Antibodies - The Human Protein Atlas

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EXOC7

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA022840

Antibody HPA022842

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA022840

HPA022842

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA022840

Antibody HPA022842

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA022840

Antibody HPA022842

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

Antibody dilution

1:20

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA022840

Antibody HPA022842

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA022842 across 45 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA022840 across 45 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 76 cell types

RNA: detected in 37 tissues
Protein: detected in 70 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA022840

Antibody HPA022842

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

83.4, 80.6, 78.8, 78.1, 77.3, 74.7, 70.2

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA022840

Antibody HPA022842

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA022840

Antibody HPA022842

Antigen

Recombinant protein fragment

Length (aa)

122

121

Antigen sequence

DDMLDVETDAYIHCVSAFVKLAQSEYQLLADIIPEHHQKKTFDSLIQDAL
DGLMLEGENIVSAARKAIVRHDFSTVLTVFPILRHLKQTKPEFDQVLQGT
AASTKNKLPGLITSMETIGAKA

DHVISYYHVASDTEKIIREGPTGRLEEYLGSMAKIQKAVEYFQDNSPDSP
ELNKVKLLFERGKEALESEFRSLMTRHSKVVSPVLILDLISGDDDLEAQE
DVTLEHLPESVLQDVIRISRW

Matching transcripts

EXOC7-001 - ENSP00000468404 [100%]
EXOC7-002 - ENSP00000384368 [100%]
EXOC7-006 - ENSP00000334100 [100%]
EXOC7-013 - ENSP00000404322 [100%]
EXOC7-018 - ENSP00000466682 [100%]
EXOC7-019 - ENSP00000333806 [100%]
EXOC7-020 - ENSP00000349767 [100%]
EXOC7-024 - ENSP00000489266 [100%]
EXOC7-201 - ENSP00000475431 [100%]

Other gene match

ANTIGEN VIEW

EXOC7-001	EXOC7-002	EXOC7-006	EXOC7-013	EXOC7-018	EXOC7-019	EXOC7-020	EXOC7-024	EXOC7-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.