PLCB1 - Antibodies - The Human Protein Atlas

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PLCB1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA034743

Antibody HPA057910

Antibody CAB004275

Antibody CAB005334

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA034743

HPA057910

sc-5291

sc-205

Host species

Rabbit

Mouse

Rabbit

Clonality

pAb

mAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Affinity

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA034743

Antibody HPA057910

Antibody CAB004275

Antibody CAB005334

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA034743

Antibody HPA057910

Antibody CAB004275

Antibody CAB005334

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

Antibody dilution

1:19

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA034743

Antibody HPA057910

Antibody CAB004275

Antibody CAB005334

Standard validation

Supported

Figure description

Immunohistochemical staining of human hippocampus shows cytoplasmic and nuclear positivity in neuronal cells.

Immunohistochemical staining of human lateral ventricle shows moderate cytoplasmic and nucleolar positivity in neuronal cells.

Immunohistochemical staining of human hippocampus shows strong cytoplasmic and nuclear positivity in neuronal cells.

Immunohistochemical staining of human hippocampus shows strong cytoplasmic and nucleolar positivity in neuronal cells.

Expression

RNA: detected in 36 tissues
Protein: detected in 32 cell types

RNA: detected in 36 tissues
Protein: detected in 9 cell types

RNA: detected in 36 tissues
Protein: detected in 25 cell types

RNA: detected in 36 tissues
Protein: detected in 7 cell types

Retrieval

HIER pH6

Antibody dilution

1:3500

1:3250

1:1300

1:1500

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA034743

Antibody HPA057910

Antibody CAB004275

Antibody CAB005334

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

138.6, 133.7, 129.6, 124.8, 109.2, 109.1

138.6, 133.7, 129.6, 124.8, 109.2, 109.1, 28, 27.7, 23.1, 22.3, 15.6, 5, 2.9

Antibody dilution

1:250

1:370

1:500

PROTEIN ARRAY

Antibody HPA034743

Antibody HPA057910

Antibody CAB004275

Antibody CAB005334

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:30000

1:4400

ANTIGEN INFORMATION

Antibody HPA034743

Antibody HPA057910

Antibody CAB004275

Antibody CAB005334

Antigen

Recombinant protein fragment

Recombinant protein

Synthetic peptide

Length (aa)

Antigen sequence

NKKKSHKSSEGSGKKKLSEQASNTYSDSSSMFEPSSPGAGEADTESDDDD
DDDDCKKSSMDEGTAGSEAMATEEMSNLVN

EEEVKKEADPGETPSEAPSEARTTPAENGVNHTTTLTPKPPSQALHSQPA
PGSVKAPAKTEDLIQSVLTEVEAQTIEELKQQKSFVK

Matching transcripts

PLCB1-003 - ENSP00000338185 [100%]
PLCB1-004 - ENSP00000367904 [100%]
PLCB1-005 - ENSP00000367908 [100%]
PLCB1-013 - ENSP00000486301 [100%]
PLCB1-014 - ENSP00000487075 [100%]
PLCB1-201 - ENSP00000479997 [100%]
PLCB1-202 - ENSP00000477664 [100%]

Other gene match

ANTIGEN VIEW

PLCB1-003	PLCB1-004	PLCB1-005	PLCB1-013	PLCB1-014	PLCB1-201	PLCB1-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.