HMCES - Antibodies - The Human Protein Atlas

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HMCES

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA044968

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA044968

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA044968

Immunocytochemistry

Approved

Immunohistochemistry

Approved

Western blot

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA044968

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.

Antibody dilution

1:101

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA044968

Standard validation

Approved

Figure description

Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Expression

RNA: detected in 37 tissues
Protein: detected in 63 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA044968

Formal validation: Genetic

Validated

Downregulation visible in both siRNA lanes

Figure description

Lane 1: Marker [kDa] 250, 150, 100, 75, 50, 37, 25, 20, 15, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled

Target mass (kDa)

40.6, 35.6, 32.1, 20.6

Loading control

Total protein image

Antibody dilution

1:250

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

40.6, 35.6, 32.1, 20.6

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA044968

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA044968

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

WRLLTMAGIFDCWEPPEGGDVLYSYTIITVDSCKGLSDIHHRMPAILDGE
EAVSKWLDFGEVSTQEALKLIHPTENITFHAVSSVVNNS

Matching transcripts

HMCES-001 - ENSP00000372955 [100%]
HMCES-002 - ENSP00000374385 [100%]
HMCES-003 - ENSP00000426215 [100%]
HMCES-004 - ENSP00000392966 [100%]
HMCES-005 - ENSP00000423132 [100%]
HMCES-006 - ENSP00000426276 [100%]

Other gene match

ANTIGEN VIEW

HMCES-001	HMCES-002	HMCES-003	HMCES-004	HMCES-005	HMCES-006

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.