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Immunohistochemical staining of human stomach (lower) shows moderate membranous positivity in glandular cells.
Immunohistochemical staining of human stomach, lower shows strong membranous and cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 17 tissues Protein: detected in 62 cell types
RNA: detected in 17 tissues Protein: detected in 23 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:60
1:150
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA054092
Antibody HPA054684
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409692)
Target mass (kDa)
36.7, 36.2
36.7, 36.2
Antibody dilution
1:130
1:250
PROTEIN ARRAY
Antibody HPA054092
Antibody HPA054684
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
