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SUMO3

ANTIBODY INFORMATION
 
 

Antibody HPA042123

  

Antibody HPA048064

  

Antibody CAB012180

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Abcam plc
  

Product name

 HPA042123   HPA048064   ab3742  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

 SUMO4 - ENSG00000177688 [100%]
SUMO2 - ENSG00000188612 [92%]
   SUMO4 - ENSG00000177688 [97%]
SUMO2 - ENSG00000188612 [100%]
    

Released in version

 12   12   4  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA042123

  

Antibody HPA048064

  

Antibody CAB012180

  
Immunocytochemistry

Approved   Approved   Supported  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Uncertain   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA042123

  

Antibody HPA048064

  

Antibody CAB012180

  

Standard validation

 Approved   Approved   Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies & cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & nuclear bodies.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.  

Antibody dilution

 1:81   1:22   1:200  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042123

  

Antibody HPA048064

  

Antibody CAB012180

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.   Immunohistochemical staining of human testis shows strong nuclear positivity in a subset of cells.   Immunohistochemical staining of human gall bladder shows strong nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 80 cell types		   RNA: detected in 37 tissues
Protein: detected in 54 cell types		   RNA: detected in 37 tissues
Protein: detected in 72 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:900   1:100   1:800  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042123

  

Antibody HPA048064

  

Antibody CAB012180

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 213, 118, 83, 50, 33, 26, 18
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 15.8, 11.6, 10.9, 10.7, 6.1   17, 15.8, 15.3, 11.6, 10.9, 10.7, 8.1   17, 15.8, 15.3, 11.6  

Antibody dilution

 1:250   1:110   1:500  

PROTEIN ARRAY
 
 

Antibody HPA042123

  

Antibody HPA048064

  

Antibody CAB012180

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:900    

ANTIGEN INFORMATION
 
 

Antibody HPA042123

  

Antibody HPA048064

  

Antibody CAB012180

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 25   32    

Antigen sequence

 
TDKPAQLEMEDEDTIDVFQQPTGGV
  
HINLKVAGQDGSVVQFKIKRHTPLSKLMKAYC
  

											 
																				

																		

										
											
Matching transcripts

												
																					SUMO3-001 - ENSP00000330343 [92%] 
SUMO3-201 - ENSP00000409666 [92%] 

											 
																						SUMO3-001 - ENSP00000330343 [100%] 
SUMO3-002 - ENSP00000380995 [100%] 
SUMO3-005 - ENSP00000380990 [100%] 
SUMO3-201 - ENSP00000409666 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					SUMO4 - ENSG00000177688 [100%]
SUMO2 - ENSG00000188612 [92%]

											 
																						SUMO4 - ENSG00000177688 [97%]
SUMO2 - ENSG00000188612 [100%]

											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SUMO3-001
SUMO3-002
SUMO3-005
SUMO3-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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