SFTPA2 - Antibodies - The Human Protein Atlas

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SFTPA2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA042638

Antibody HPA045752

Antibody HPA049368

Antibody CAB002439

Provider

Atlas Antibodies
Sigma-Aldrich

Novocastra

Product name

HPA042638

HPA045752

HPA049368

NCL-SP-A

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Other gene match

SFTPA1 - ENSG00000122852 [100%]

Released in version

References

VALIDATION SUMMARY

Antibody HPA042638

Antibody HPA045752

Antibody HPA049368

Antibody CAB002439

Immunohistochemistry

Western blot

Protein array

IMMUNOHISTOCHEMISTRY

Antibody HPA042638

Antibody HPA045752

Antibody HPA049368

Antibody CAB002439

Standard validation

Supported

Figure description

Immunohistochemical staining of human lung shows strong cytoplasmic and membranous positivity in pneumocytes.

Immunohistochemical staining of human lung shows strong cytoplasmic positivity in a fraction of pneumocytes.

Immunohistochemical staining of human lung shows strong cytoplasmic positivity in alveolar cells

Expression

RNA: detected in 12 tissues
Protein: detected in 3 cell types

RNA: detected in 12 tissues
Protein: detected in 15 cell types

RNA: detected in 12 tissues
Protein: detected in 2 cell types

Retrieval

HIER pH6

Antibody dilution

1:200

1:2000

1:175

1:200

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

Mainly consistent with RNA expression data.

Mainly not consistent with RNA expression data.

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA042638

Antibody HPA045752

Antibody HPA049368

Antibody CAB002439

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

27.7, 26.2, 16, 15.9

27.7, 26.2

26.2, 15.9, 9.4

Antibody dilution

1:250

1:860

1:350

1:500

PROTEIN ARRAY

Antibody HPA042638

Antibody HPA045752

Antibody HPA049368

Antibody CAB002439

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:6900

1:30000

ANTIGEN INFORMATION

Antibody HPA042638

Antibody HPA045752

Antibody HPA049368

Antibody CAB002439

Antigen

Recombinant protein fragment

Not known

Length (aa)

Antigen sequence

PGLPAHLDEELQATLHDFRHQILQTRGALSLQGSIMTVGEKVFSSNGQSI
TFDAIQEACARA

IAVPRNPEENEAIASFVKKYNTYAYVGLTEGPSPGDFRYSDGTPVNYTNW
YRG

KEQCVEMYTDGQWNDRNCLYSRLTICE

Matching transcripts

SFTPA2-001 - ENSP00000361400 [100%]
SFTPA2-003 - ENSP00000397375 [100%]
SFTPA2-201 - ENSP00000361402 [100%]

SFTPA2-001 - ENSP00000361400 [100%]
SFTPA2-201 - ENSP00000361402 [100%]

Other gene match

SFTPA1 - ENSG00000122852 [100%]

ANTIGEN VIEW

SFTPA2-001	SFTPA2-003	SFTPA2-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.