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Immunofluorescent staining of human cell line SH-SY5Y shows localization to the Golgi apparatus.
Antibody dilution
1:169
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA053879
Antibody HPA062262
Antibody CAB005872
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human placenta shows positivity in vessels.
Immunohistochemical staining of human placenta shows positivity in endothelial cells.
Immunohistochemical staining of human placenta shows distinct positivity in endothelial cells.
Expression
RNA: detected in 20 tissues Protein: detected in 4 cell types
RNA: detected in 20 tissues Protein: detected in 1 cell types
RNA: detected in 20 tissues Protein: detected in 15 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:40
1:2000
1:1500
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA053879
Antibody HPA062262
Antibody CAB005872
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
41.3, 33.1
41.3
41.3, 33.1, 14.5, 10.3
Antibody dilution
1:90
1:560
1:500
PROTEIN ARRAY
Antibody HPA053879
Antibody HPA062262
Antibody CAB005872
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:700
1:6750
ANTIGEN INFORMATION
Antibody HPA053879
Antibody HPA062262
Antibody CAB005872
Antigen
Recombinant protein fragment
Recombinant protein fragment
Synthetic peptide
Length (aa)
52
65
Antigen sequence
LNKCETWVSNLRYNHMLRKKKNLLLQYNSGEDLAVNIIFPEKIDMTTFSK
EA
