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ANTIBODY INFORMATION
 
 

Antibody HPA001853

  

Antibody HPA012292

  

Antibody CAB000330

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA001853   HPA012292   sc-14027  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 3   4   1  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA001853

  

Antibody HPA012292

  

Antibody CAB000330

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001853

  

Antibody HPA012292

  

Antibody CAB000330

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA012292.		  
Validated

Antibody staining overlaps with antibody HPA001853.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.    

Antibody dilution

 1:14   1:5    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001853

  

Antibody HPA012292

  

Antibody CAB000330

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human small intestine shows nuclear positivity in glandular cells.   Immunohistochemical staining of human small intestine shows nuclear positivity in glandular cells.   Immunohistochemical staining of human small intestine shows nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 78 cell types		   RNA: detected in 37 tissues
Protein: detected in 58 cell types		   RNA: detected in 37 tissues
Protein: detected in 58 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:25   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001853

  

Antibody HPA012292

  

Antibody CAB000330

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 80.7, 79.9   80.7, 79.9, 23   80.7, 79.9, 23, 16.1  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001853

  

Antibody HPA012292

  

Antibody CAB000330

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA001853

  

Antibody HPA012292

  

Antibody CAB000330

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 147   115    

Antigen sequence

 
QVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQTSSSNTTLTPIASAAS
IPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHPIQGLPLAIANAPGD
HGAQLGLHGAGGDGIHDDTAGGEEGENSPDAQPQAGRRTRREACTCP
  
NGGGAFSQARSSSTGSSSSTGGGGQESQPSPLALLAATCSRIESPNENSN
NSQGPSQSGGTGELDLTATQLSQGANGWQIISSSSGATPTSKEQSGSSTN
GSNGSESSKNRTVSG
  

											 
																				

																		

										
											
Matching transcripts

												
																					SP1-001 - ENSP00000329357 [100%] 
SP1-002 - ENSP00000404263 [100%] 

											 
																						SP1-001 - ENSP00000329357 [100%] 
SP1-002 - ENSP00000404263 [100%] 
SP1-004 - ENSP00000458133 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SP1-001
SP1-002
SP1-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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