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PBX1

ANTIBODY INFORMATION
 
 

Antibody HPA003505

  

Antibody HPA003881

  

Antibody CAB018768

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA003505   HPA003881   sc-28313  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

   PBX3 - ENSG00000167081 [81%]
    

Released in version

 4   4   4  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA003505

  

Antibody HPA003881

  

Antibody CAB018768

  
Immunocytochemistry

Supported   Approved   Supported  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003505

  

Antibody HPA003881

  

Antibody CAB018768

  

Standard validation

 Supported   Approved   Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.  

Antibody dilution

 1:42   1:10   1:13  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is partly supported by literature or no literature is available.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003505

  

Antibody HPA003881

  

Antibody CAB018768

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.   Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.   Immunohistochemical staining of human fallopian tube shows nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 55 cell types		   RNA: detected in 37 tissues
Protein: detected in 51 cell types		   RNA: detected in 37 tissues
Protein: detected in 45 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:50   1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003505

  

Antibody HPA003881

  

Antibody CAB018768

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 46.6, 46.3, 38.4, 37, 18.2   47.2, 46.6, 46.3, 39.2, 37.1, 35.2   46.6, 46.3, 38.4, 37.1, 37, 35.2, 28.9, 18.2, 17.6, 15.6, 14.3, 10.5, 7.4  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA003505

  

Antibody HPA003881

  

Antibody CAB018768

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:2000    

ANTIGEN INFORMATION
 
 

Antibody HPA003505

  

Antibody HPA003881

  

Antibody CAB018768

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 113   144    

Antigen sequence

 
PRLMHSHAGVGMAGHPGLSQHLQDGAGGTEGEGGRKQDIGDILQQIMTIT
DQSLDEAQARKHALNCHRMKPALFNVLCEIKEKTVLSIRGAQEEEPTDPQ
LMRLDNMLLAEGV
  
YSHLSNPYPSEEAKEELAKKCGITVSQVSNWFGNKRIRYKKNIGKFQEEA
NIYAAKTAVTATNVSAHGSQANSPSTPNSAGSSSSFNMSNSGDLFMSVQS
LNGDSYQGAQVGANVQSQVDTLRHVISQTGGYSDGLAASQMYSP
   

Matching transcripts

 PBX1-001 - ENSP00000405890 [100%]
PBX1-002 - ENSP00000356872 [100%]
PBX1-007 - ENSP00000341455 [100%]
PBX1-012 - ENSP00000453188 [100%]
PBX1-020 - ENSP00000485692 [100%]
  PBX1-001 - ENSP00000405890 [100%]
PBX1-017 - ENSP00000452727 [100%]
PBX1-020 - ENSP00000485692 [100%]
PBX1-201 - ENSP00000439943 [100%]
   

Other gene match

   PBX3 - ENSG00000167081 [81%]
    
ANTIGEN VIEW
PBX1-001
PBX1-002
PBX1-007
PBX1-012
PBX1-017
PBX1-020
PBX1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.