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BCAP31

ANTIBODY INFORMATION
 
 

Antibody HPA003906

  

Antibody CAB015350

  

Antibody CAB015424

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Santa Cruz Biotechnology
  

Product name

 HPA003906   sc-48766   sc-56008  

Host species

 Rabbit   Rabbit   Rat  

Clonality

 pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

      

Released in version

 2   4   4  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA003906

  

Antibody CAB015350

  

Antibody CAB015424

  
Immunocytochemistry

Supported   Supported    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Approved      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003906

  

Antibody CAB015350

  

Antibody CAB015424

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.   Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.    

Antibody dilution

 1:200   1:200    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003906

  

Antibody CAB015350

  

Antibody CAB015424

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.   Immunohistochemical staining of human pancreas shows cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human pancreas shows cytoplasmic positivity in exocrine glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 77 cell types		   RNA: detected in 37 tissues
Protein: detected in 65 cell types		   RNA: detected in 37 tissues
Protein: detected in 45 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:6000   1:800   1:35  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  
 

Standard validation

 Approved      

     

Figure description

 Synaptic positivity was observed in most regions of the brain.      

WESTERN BLOT
 
 

Antibody HPA003906

  

Antibody CAB015350

  

Antibody CAB015424

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 34.8, 28   34.8, 28, 21.3, 21.2, 20.7, 20.2, 16   34.8, 28, 21.3, 21.2, 20.7, 20.2, 16  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA003906

  

Antibody CAB015350

  

Antibody CAB015424

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA003906

  

Antibody CAB015350

  

Antibody CAB015424

  

Antigen

 Recombinant protein fragment   Recombinant protein   Not known  

Length (aa)

 100      

Antigen sequence

 
ESASEAAKKYMEENDQLKKGAAVDGGKLDVGNAEVKLEEENRSLKADLQK
LKDELASTKQKLEKAENQVLAMRKQSEGLTKEYDRLLEEHAKLQAAVDGP
     

Matching transcripts

 BCAP31-001 - ENSP00000343458 [100%]
BCAP31-002 - ENSP00000392330 [100%]
     

Other gene match

      
ANTIGEN VIEW
BCAP31-001
BCAP31-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.