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SPATC1

ANTIBODY INFORMATION
 
 

Antibody HPA039590

  

Antibody HPA040220

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA039590   HPA040220  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   9  

References

   1  

VALIDATION SUMMARY
 
 

Antibody HPA039590

  

Antibody HPA040220

  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039590

  

Antibody HPA040220

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human duodenum shows strong membranous positivity in glandular cells.   Immunohistochemical staining of human stomach, lower shows strong membranous and cytoplasmic positivity in gastric parietal cells.  

Expression

 RNA: detected in 4 tissues
Protein: detected in 39 cell types		   RNA: detected in 4 tissues
Protein: detected in 61 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:60   1:60  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA039590

  

Antibody HPA040220

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 62.4, 45.4   62.4  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA039590

  

Antibody HPA040220

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA039590

  

Antibody HPA040220

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 77   73  

Antigen sequence

 
LIAPVMGTVAVSLSSPLLSSTATPPGVSQNLLANPMSNLVLPEAPRLRLA
EPLRGGPTGPQSPACVVPTATTKVPLS
  
PPRTSSSPASVNDSRGPRTTEPSTKSMMEVERKLAHRKTSKFPENPRESK
QLAWERLVGEIAFQLDRRILSSI
 

Matching transcripts

 SPATC1-001 - ENSP00000366690 [100%]
SPATC1-002 - ENSP00000387613 [100%]
  SPATC1-001 - ENSP00000366690 [100%]
 

Other gene match

    
ANTIGEN VIEW
SPATC1-001
SPATC1-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA039590

  

Antibody HPA040220

  

1.

   The transcriptomic and proteomic landscapes of bone marrow and secondary lymphoid tissues
Andersson S et al
PLoS One 2014;9(12):e115911
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.