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ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA012692

 

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA012692  

Host species

Rabbit  

Clonality

pAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 

Released in version

4  

References

1  

VALIDATION SUMMARY

 
 

Antibody HPA012692

 
Immunohistochemistry

Uncertain  
Western blot

Supported  
Protein array

Approved  

IMMUNOHISTOCHEMISTRY

 
 

Antibody HPA012692

 

Standard validation

Uncertain  

 

Figure description

Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells and moderate cytoplasmic positivity in decidual cells.  

Expression

RNA: detected in 0 tissues
Protein: detected in 57 cell types
 

Retrieval

HIER pH6  

Antibody dilution

1:250  

Literature conformity

No avaliable gene/protein characterization data.  

RNA consistency

Not consistent with RNA expression data.  
 

Standard validation

Approved  

 

Figure description

Weak labeling of synaptic fields in the cerebellum and hippocampus.  

WESTERN BLOT

 
 

Antibody HPA012692

 

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.
 

 

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
 

Target mass (kDa)

26.9  

Antibody dilution

1:250  

PROTEIN ARRAY

 
 

Antibody HPA012692

 

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
 

 

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

1:500  

ANTIGEN INFORMATION

 
 

Antibody HPA012692

 

Antigen

Recombinant protein fragment  

Length (aa)

122  

Antigen sequence

RGRLYASEHYSAECEFVERIHELGYNTYASRLYRTVSSTPGARRQPSAER
LWYVSVNGKGRPRRGFKTRRTQKSSLFLPRVLDHRDHEMVRQLQSGLPRP
PGKGVQPRRRRQKQSPDNLEPS
 

Matching transcripts

FGF3-001 - ENSP00000334122 [100%]
 

Other gene match

 
ANTIGEN VIEW
FGF3-001

RELEVANT PUBLICATIONS

 
 

Antibody HPA012692

 

1.

Mitochondrial mass, a new metabolic biomarker for stem-like cancer cells: Understanding WNT/FGF-driven anabolic signaling
Lamb R et al
Oncotarget 2015;6(31):30453-71
Application: WB
 
 

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