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Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center and non-germinal center cells.
Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in a subset of germinal centre cells of lymphoid tissue.
Expression
RNA: detected in 6 tissues Protein: detected in 9 cell types
RNA: detected in 6 tissues Protein: detected in 29 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:100
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035981
Antibody CAB038418
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401555)
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
31.6
31.6, 13.8
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA035981
Antibody CAB038418
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
