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H2AFX

ANTIBODY INFORMATION
 
 

Antibody HPA041189

  

Antibody HPA051647

  

Antibody CAB012264

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Abcam plc
  

Product name

 HPA041189   HPA051647   ab10475  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

 H2AFY2 - ENSG00000099284 [81%]
H2AFY - ENSG00000113648 [85%]
HIST1H2AA - ENSG00000164508 [96%]
HIST1H2AC - ENSG00000180573 [92%]
HIST3H2A - ENSG00000181218 [92%]
HIST2H2AC - ENSG00000184260 [92%]
HIST2H2AB - ENSG00000184270 [100%]
HIST1H2AI - ENSG00000196747 [92%]
HIST1H2AG - ENSG00000196787 [92%]
HIST1H2AD - ENSG00000196866 [92%]
HIST2H2AA3 - ENSG00000203812 [92%]
H2AFJ - ENSG00000246705 [92%]
HIST2H2AA4 - ENSG00000272196 [92%]
HIST1H2AH - ENSG00000274997 [92%]
HIST1H2AK - ENSG00000275221 [92%]
HIST1H2AJ - ENSG00000276368 [92%]
HIST1H2AL - ENSG00000276903 [92%]
HIST1H2AE - ENSG00000277075 [92%]
HIST1H2AB - ENSG00000278463 [92%]
HIST1H2AM - ENSG00000278677 [92%]
RP1-34B20.21 - ENSG00000282988 [92%]
      

Released in version

 9   14   4  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA041189

  

Antibody HPA051647

  

Antibody CAB012264

  
Immunocytochemistry

  Supported    
Immunohistochemistry

Supported     Supported  
Western blot

Uncertain   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041189

  

Antibody HPA051647

  

Antibody CAB012264

  

Standard validation

   Supported    

     

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear speckles.    

Antibody dilution

   1:7    

Literature conformity

   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041189

  

Antibody HPA051647

  

Antibody CAB012264

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.     Immunohistochemical staining of human cerebellum shows strong nuclear positivity in cerebellar tissue.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 75 cell types		     RNA: detected in 37 tissues
Protein: detected in 78 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:500     1:500  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041189

  

Antibody HPA051647

  

Antibody CAB012264

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 213, 118, 83, 50, 33, 26, 18
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 40.1, 39.6, 39.5, 39.2, 18.5, 15.1, 14.2, 14.1, 14, 13.9   15.1   15.1  

Antibody dilution

 1:250   1:80   1:500  

PROTEIN ARRAY
 
 

Antibody HPA041189

  

Antibody HPA051647

  

Antibody CAB012264

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:550    

ANTIGEN INFORMATION
 
 

Antibody HPA041189

  

Antibody HPA051647

  

Antibody CAB012264

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 26   26    

Antigen sequence

 
AVRNDEELNKLLGGVTIAQGGVLPNI
  
PKKTSATVGPKAPSGGKKATQASQEY
  

											 
																				

																		

										
											
Matching transcripts

												
																					H2AFX-001 - ENSP00000434024 [96%] 

											 
																						H2AFX-001 - ENSP00000434024 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					H2AFY2 - ENSG00000099284 [81%]
H2AFY - ENSG00000113648 [85%]
HIST1H2AA - ENSG00000164508 [96%]
HIST1H2AC - ENSG00000180573 [92%]
HIST3H2A - ENSG00000181218 [92%]
HIST2H2AC - ENSG00000184260 [92%]
HIST2H2AB - ENSG00000184270 [100%]
HIST1H2AI - ENSG00000196747 [92%]
HIST1H2AG - ENSG00000196787 [92%]
HIST1H2AD - ENSG00000196866 [92%]
HIST2H2AA3 - ENSG00000203812 [92%]
H2AFJ - ENSG00000246705 [92%]
HIST2H2AA4 - ENSG00000272196 [92%]
HIST1H2AH - ENSG00000274997 [92%]
HIST1H2AK - ENSG00000275221 [92%]
HIST1H2AJ - ENSG00000276368 [92%]
HIST1H2AL - ENSG00000276903 [92%]
HIST1H2AE - ENSG00000277075 [92%]
HIST1H2AB - ENSG00000278463 [92%]
HIST1H2AM - ENSG00000278677 [92%]
RP1-34B20.21 - ENSG00000282988 [92%]

											 
																						
											 
																						
											 
																				

																		

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