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H2AFX

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GENERAL INFORMATION

Gene name

 H2AFX

Gene description

 H2A histone family, member X

Protein class

 Plasma proteins
Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 1
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HUMAN PROTEIN ATLAS INFORMATION

Summary

 Mainly localized to the nucleoplasm. In addition localized to the nuclear speckles.

RNA cell category

 Expressed in all

Protein evidence

 Evidence at protein level

Main location

 Localized to the Nucleoplasm (supported)

Additional location

 In addition localized to the Nuclear speckles (supported)

Single-cell variation

 Single-cell variation in protein expression observed.

Cell cycle dependency

 Cell cycle dependent expression determined by custom assay

Mouse

 Localized to the Nucleoplasm in mouse cells.

DATA RELIABILITY

Reliability score

 Supported based on 1 antibody.
HPA051647
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ASSAYS
RNA expression
Human cells
Custom data
Mouse cells

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all
Organ
Origin
Category
Expression
Alphabetical
Cell lines sorted after organ of phenotypic resemblance.

HUMAN CELLS

Summary

 Mainly localized to the nucleoplasm. In addition localized to the nuclear speckles.

Main location

 Nucleoplasm (supported)

Additional location

 Nuclear speckles (supported)

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

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Antibody

Cell line

Location

Single-cell variation

Cell cycle dependent variation
HPA051647 A-431 Nucleoplasm
Nuclear speckles Intensity
HPA051647 U-2 OS Nucleoplasm Intensity Intensity
HPA051647 U-251 MG Nucleoplasm
Nuclear speckles Intensity

CUSTOM DATA

 

 

 
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Antibody

Cell line

Assay

Description
HPA051647 U-2 OS Image Staining of U-2 OS FUCCI cells, to characterize the cell cycle dependency of the protein expression pattern. The FUCCI cells, Fluorescence Ubiquitination Cell Cycle Indicator, are cells tagged with different fluorescent proteins fused to two different cell cycle regulators Cdt1 (expressed in G1 phase) and Geminin (expressed in S and G2 phases) that allows cell cycle monitoring. When both proteins are present, the overlay of the images appear in yellow marking the G1/S transition. H2AFX is mostly expressed at the G1/S checkpoint, and during S and/or G2.

CELL CYCLE

Summary

 Expression has cell cycle dependency according to custom assay

Intensity variation

 Nuclear speckles, Nucleoplasm

MOUSE CELLS

Main location

 Nucleoplasm

Mouse gene

 ENSMUSG00000049932 (version 83)

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

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Antibody

Cell line

Location
HPA051647 NIH 3T3 Nucleoplasm

GENE INFORMATION

Gene name

 H2AFX (HGNC Symbol)

Synonyms

 H2AX

Description

 H2A histone family, member X (HGNC Symbol)

Entrez gene summary

 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]

Chromosome

 11

Cytoband

 q23.3

Chromosome location (bp)

 119093854 - 119095467

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000188486 (version 83.38)

Entrez gene

 3014

UniProt

 P16104 (UniProt - Evidence at protein level)

neXtProt

 NX_P16104

Antibodypedia

 H2AFX antibodies

PROTEIN BROWSER
H2AFX-001

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
H2AFX-001
ENSP00000434024
ENST00000530167
P16104 [Direct mapping]
Histone H2AX
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   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Plasma proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000077 [DNA damage checkpoint]
GO:0000724 [double-strand break repair via homologous recombination]
GO:0000781 [chromosome, telomeric region]
GO:0000785 [chromatin]
GO:0000786 [nucleosome]
GO:0000790 [nuclear chromatin]
GO:0000794 [condensed nuclear chromosome]
GO:0001673 [male germ cell nucleus]
GO:0001741 [XY body]
GO:0003677 [DNA binding]
GO:0003684 [damaged DNA binding]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005657 [replication fork]
GO:0005694 [chromosome]
GO:0006281 [DNA repair]
GO:0006302 [double-strand break repair]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0006325 [chromatin organization]
GO:0006334 [nucleosome assembly]
GO:0006342 [chromatin silencing]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0007283 [spermatogenesis]
GO:0010212 [response to ionizing radiation]
GO:0016032 [viral process]
GO:0019899 [enzyme binding]
GO:0021987 [cerebral cortex development]
GO:0035861 [site of double-strand break]
GO:0042393 [histone binding]
GO:0045739 [positive regulation of DNA repair]
GO:0046982 [protein heterodimerization activity]
GO:0051321 [meiotic cell cycle]
GO:0070062 [extracellular exosome]
GO:0071480 [cellular response to gamma radiation]
GO:0090398 [cellular senescence]
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 143 aa
15.1 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.