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GREB1

ANTIBODY INFORMATION
 
 

Antibody HPA024616

  

Antibody HPA027843

  

Antibody HPA028416

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA024616   HPA027843   HPA028416  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 6   6   6  

References

 1      

VALIDATION SUMMARY
 
 

Antibody HPA024616

  

Antibody HPA027843

  

Antibody HPA028416

  
Immunocytochemistry

Approved      
Immunohistochemistry

Uncertain   Uncertain   Uncertain  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA024616

  

Antibody HPA027843

  

Antibody HPA028416

  

Standard validation

 Approved      

     

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.      

Antibody dilution

 1:52      

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA024616

  

Antibody HPA027843

  

Antibody HPA028416

  

Standard validation

 Uncertain   Uncertain   Uncertain  

     

Figure description

 Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.   Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 22 tissues
Protein: detected in 4 cell types		   RNA: detected in 22 tissues
Protein: detected in 47 cell types		   RNA: detected in 22 tissues
Protein: detected in 45 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:75   1:13  

Literature conformity

 Not consistent with gene/protein characterization data.   Not consistent with gene/protein characterization data.   Not consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA024616

  

Antibody HPA027843

  

Antibody HPA028416

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 216.5, 60.2   216.5, 60.2   216.5, 107.2  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA024616

  

Antibody HPA027843

  

Antibody HPA028416

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA024616

  

Antibody HPA027843

  

Antibody HPA028416

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 93   87   90  

Antigen sequence

 
EGDIDILLDKFHQENQGHISSSLAASSVTKAASLDVSGTPVCTSYNLEPH
SIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIP
  
SSCNDSVHVIECAYSLAEGLSEMFRLLVEGKLAKTNYVVIICACRSAAID
SCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKV
  
RKWSSKTRASEVQEPFSRCHVHNFIILNVDLTQNVQYNQNRFLCDDVDFN
LRVHSAGLLLCRFNRFSVMKKQIVVGGHRSFHITSKVSDN
 

Matching transcripts

 GREB1-001 - ENSP00000370896 [100%]
GREB1-005 - ENSP00000403886 [100%]
GREB1-201 - ENSP00000234142 [100%]
  GREB1-001 - ENSP00000370896 [100%]
GREB1-005 - ENSP00000403886 [100%]
GREB1-201 - ENSP00000234142 [100%]
  GREB1-001 - ENSP00000370896 [100%]
GREB1-006 - ENSP00000379429 [100%]
GREB1-201 - ENSP00000234142 [100%]
 

Other gene match

      
ANTIGEN VIEW
GREB1-001
GREB1-005
GREB1-006
GREB1-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA024616

  

Antibody HPA027843

  

Antibody HPA028416

  

1.

 17β-estradiol upregulates GREB1 and accelerates ovarian tumor progression in vivo
Laviolette LA et al
Int J Cancer 2014;135(5):1072-84
      
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.