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Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies.
Antibody dilution
1:49
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA011548
Antibody HPA048032
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human liver shows strong cytoplasmic and membranous positivity in bile duct cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells, endothelial cells were moderately stained.
Expression
RNA: detected in 25 tissues Protein: detected in 30 cell types
RNA: detected in 25 tissues Protein: detected in 77 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:30
1:55
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA011548
Antibody HPA048032
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
37, 25.8
37
Antibody dilution
1:250
1:240
PROTEIN ARRAY
Antibody HPA011548
Antibody HPA048032
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:500
1:500
ANTIGEN INFORMATION
Antibody HPA011548
Antibody HPA048032
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
101
130
Antigen sequence
PGPIRWFQGAGLSREAIYNFGGISHPKATAVQASNNDFSILLQNVSSEDA
GTYYCVKFQRKPNRQYLSGQGTSLKVKAKSTSSKEAEFTSEPATEMSPTG
L
