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Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & endoplasmic reticulum.
Antibody dilution
1:87
1:8
1:125
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA012542
Antibody CAB009329
Antibody CAB015217
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in both reaction center cells and lymphoid cells outside reaction centra.
Expression
RNA: detected in 37 tissues Protein: detected in 69 cell types
RNA: detected in 37 tissues Protein: detected in 65 cell types
RNA: detected in 37 tissues Protein: detected in 43 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:125
1:30
1:500
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA012542
Antibody CAB009329
Antibody CAB015217
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
50, 41.3
50, 41.3
50, 41.3
Antibody dilution
1:250
1:500
1:500
PROTEIN ARRAY
Antibody HPA012542
Antibody CAB009329
Antibody CAB015217
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
