Fields »
ABOUT
HELP
BLOG
XRCC6

Please wait until image is loaded before changing channels

GENERAL INFORMATION

Gene name

 XRCC6

Gene description

 X-ray repair complementing defective repair in Chinese hamster cells 6

Protein class

 Cancer-related genes
Plasma proteins
Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 6
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

Summary

 Localized to the nucleoplasm.

RNA cell category

 Expressed in all

Protein evidence

 Evidence at protein level

Main location

 Localized to the Nucleoplasm (validated)

Single-cell variation

 Single-cell variation in protein expression observed.

siRNA

 The location(s) are Validated based on siRNA mediated gene silencing.

GFP

 The location(s) are Validated based on analysis of GFP-tagged protein.

Mouse

 Localized to the Nucleus in mouse cells.

DATA RELIABILITY

Reliability score

 Validated based on 2 antibodies.
HPA047549 , HPA062226
SHOW MORE

ASSAYS
RNA expression
Human cells
siRNA
GFP
Mouse cells

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all
Organ
Origin
Category
Expression
Alphabetical
Cell lines sorted after organ of phenotypic resemblance.

HUMAN CELLS

Summary

 Localized to the nucleoplasm.

Main location

 Nucleoplasm (validated)

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

Location

Single-cell variation

Cell cycle dependent variation
HPA047549 HEK 293 Nucleoplasm
HPA047549 PC-3 Nucleoplasm
HPA047549 U-2 OS Nucleoplasm Intensity
HPA062226 HEK 293 Nucleoplasm
HPA062226 RH-30 Nucleoplasm
HPA062226 U-2 OS Nucleoplasm

siRNA VALIDATION
 

Relative fluorescence intensity (RFI)

Antibody

 HPA047549

siRNA validation

 Validated: Significant downregulation > 25% by both siRNA:s.

Analysis performed in

 Nuclear region of segmented cells in 10x-images

siRNA 1

siRNA 2

RFI

 40%37%

p-value

 < 0.01< 0.01
 

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail 10x

Thumbnail 40x

Antibody

Sample

Cell line
HPA047549 siRNA 2 U-2 OS
HPA047549 scrambled U-2 OS
HPA047549 siRNA 1 U-2 OS

GFP

Antibody

 HPA047549

Validation

C-terminal tag
BAC cell line: 3840
Validated: Antibody staining overlaps with GFP tagged protein

Antibody

 HPA062226

Validation

C-terminal tag
BAC cell line: 3840
Validated: Antibody staining overlaps with GFP tagged protein

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

BAC id

Tag

Location antibody staining

Location tagged protein
HPA047549 HeLa 3840 C-terminal Nucleoplasm Nucleoplasm
HPA047549 HeLa Normal - Nucleoplasm -
HPA062226 HeLa 3840 C-terminal Nucleoplasm Nucleoplasm
HPA062226 HeLa Normal - Nucleoplasm -

MOUSE CELLS

Main location

 Nucleus

Mouse gene

 ENSMUSG00000022471 (version 83)

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

Location
HPA047549 NIH 3T3 Nucleus

GENE INFORMATION

Gene name

 XRCC6 (HGNC Symbol)

Synonyms

 D22S671, D22S731, G22P1, KU70, ML8

Description

 X-ray repair complementing defective repair in Chinese hamster cells 6 (HGNC Symbol)

Entrez gene summary

 The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]

Chromosome

 22

Cytoband

 q13.2

Chromosome location (bp)

 41621119 - 41664048

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000196419 (version 83.38)

Entrez gene

 2547

UniProt

 P12956 (UniProt - Evidence at protein level)

neXtProt

 NX_P12956

Antibodypedia

 XRCC6 antibodies

PROTEIN BROWSER
XRCC6-001
XRCC6-002
XRCC6-003
XRCC6-005
XRCC6-006
XRCC6-201

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
XRCC6-001
ENSP00000353192
ENST00000360079
P12956 [Direct mapping]
X-ray repair cross-complementing protein 6
A0A024R1N4 [Target identity:100%; Query identity:100%]
X-ray repair complementing defective repair in Chinese hamster cells 6 (Ku autoantigen, 70kDa), isoform CRA_a
Show all
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000723 [telomere maintenance]
GO:0000783 [nuclear telomere cap complex]
GO:0000784 [nuclear chromosome, telomeric region]
GO:0003677 [DNA binding]
GO:0003684 [damaged DNA binding]
GO:0003690 [double-stranded DNA binding]
GO:0003691 [double-stranded telomeric DNA binding]
GO:0004003 [ATP-dependent DNA helicase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005667 [transcription factor complex]
GO:0005829 [cytosol]
GO:0006266 [DNA ligation]
GO:0006281 [DNA repair]
GO:0006302 [double-strand break repair]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0006310 [DNA recombination]
GO:0006351 [transcription, DNA-templated]
GO:0008022 [protein C-terminus binding]
GO:0016020 [membrane]
GO:0016032 [viral process]
GO:0032481 [positive regulation of type I interferon production]
GO:0032508 [DNA duplex unwinding]
GO:0042162 [telomeric DNA binding]
GO:0043564 [Ku70:Ku80 complex]
GO:0044212 [transcription regulatory region DNA binding]
GO:0044822 [poly(A) RNA binding]
GO:0045087 [innate immune response]
GO:0045892 [negative regulation of transcription, DNA-templated]
GO:0045893 [positive regulation of transcription, DNA-templated]
GO:0045944 [positive regulation of transcription from RNA polymerase II promoter]
GO:0048660 [regulation of smooth muscle cell proliferation]
GO:0051575 [5'-deoxyribose-5-phosphate lyase activity]
GO:0070419 [nonhomologous end joining complex]
GO:0071480 [cellular response to gamma radiation]
GO:0071481 [cellular response to X-ray]
GO:0075713 [establishment of integrated proviral latency]
Show all
 609 aa
69.8 kDa 		No 0
XRCC6-002
ENSP00000384941
ENST00000402580
P12956 [Direct mapping]
X-ray repair cross-complementing protein 6
Show all
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000723 [telomere maintenance]
GO:0000783 [nuclear telomere cap complex]
GO:0000784 [nuclear chromosome, telomeric region]
GO:0003677 [DNA binding]
GO:0003684 [damaged DNA binding]
GO:0003690 [double-stranded DNA binding]
GO:0003691 [double-stranded telomeric DNA binding]
GO:0004003 [ATP-dependent DNA helicase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005667 [transcription factor complex]
GO:0005829 [cytosol]
GO:0006266 [DNA ligation]
GO:0006281 [DNA repair]
GO:0006302 [double-strand break repair]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0006310 [DNA recombination]
GO:0006351 [transcription, DNA-templated]
GO:0008022 [protein C-terminus binding]
GO:0016020 [membrane]
GO:0016032 [viral process]
GO:0032481 [positive regulation of type I interferon production]
GO:0032508 [DNA duplex unwinding]
GO:0042162 [telomeric DNA binding]
GO:0043564 [Ku70:Ku80 complex]
GO:0044212 [transcription regulatory region DNA binding]
GO:0044822 [poly(A) RNA binding]
GO:0045087 [innate immune response]
GO:0045892 [negative regulation of transcription, DNA-templated]
GO:0045893 [positive regulation of transcription, DNA-templated]
GO:0045944 [positive regulation of transcription from RNA polymerase II promoter]
GO:0048660 [regulation of smooth muscle cell proliferation]
GO:0051575 [5'-deoxyribose-5-phosphate lyase activity]
GO:0070419 [nonhomologous end joining complex]
GO:0071480 [cellular response to gamma radiation]
GO:0071481 [cellular response to X-ray]
GO:0075713 [establishment of integrated proviral latency]
Show all
 568 aa
65.1 kDa 		No 0
XRCC6-003
ENSP00000352257
ENST00000359308
P12956 [Direct mapping]
X-ray repair cross-complementing protein 6
A0A024R1N4 [Target identity:100%; Query identity:100%]
X-ray repair complementing defective repair in Chinese hamster cells 6 (Ku autoantigen, 70kDa), isoform CRA_a
Show all
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000723 [telomere maintenance]
GO:0000783 [nuclear telomere cap complex]
GO:0000784 [nuclear chromosome, telomeric region]
GO:0003677 [DNA binding]
GO:0003684 [damaged DNA binding]
GO:0003690 [double-stranded DNA binding]
GO:0003691 [double-stranded telomeric DNA binding]
GO:0004003 [ATP-dependent DNA helicase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005667 [transcription factor complex]
GO:0005829 [cytosol]
GO:0006266 [DNA ligation]
GO:0006281 [DNA repair]
GO:0006302 [double-strand break repair]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0006310 [DNA recombination]
GO:0006351 [transcription, DNA-templated]
GO:0008022 [protein C-terminus binding]
GO:0016020 [membrane]
GO:0016032 [viral process]
GO:0032481 [positive regulation of type I interferon production]
GO:0032508 [DNA duplex unwinding]
GO:0042162 [telomeric DNA binding]
GO:0043564 [Ku70:Ku80 complex]
GO:0044212 [transcription regulatory region DNA binding]
GO:0044822 [poly(A) RNA binding]
GO:0045087 [innate immune response]
GO:0045892 [negative regulation of transcription, DNA-templated]
GO:0045893 [positive regulation of transcription, DNA-templated]
GO:0045944 [positive regulation of transcription from RNA polymerase II promoter]
GO:0048660 [regulation of smooth muscle cell proliferation]
GO:0051575 [5'-deoxyribose-5-phosphate lyase activity]
GO:0070419 [nonhomologous end joining complex]
GO:0071480 [cellular response to gamma radiation]
GO:0071481 [cellular response to X-ray]
GO:0075713 [establishment of integrated proviral latency]
Show all
 609 aa
69.8 kDa 		No 0
XRCC6-005
ENSP00000384082
ENST00000405506
B1AHC9 [Direct mapping]
X-ray repair cross-complementing protein 6
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000723 [telomere maintenance]
GO:0003677 [DNA binding]
GO:0003684 [damaged DNA binding]
GO:0004003 [ATP-dependent DNA helicase activity]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0032508 [DNA duplex unwinding]
GO:0042162 [telomeric DNA binding]
GO:0043564 [Ku70:Ku80 complex]
Show all
 559 aa
64.3 kDa 		No 0
XRCC6-006
ENSP00000384257
ENST00000405878
P12956 [Direct mapping]
X-ray repair cross-complementing protein 6
A0A024R1N4 [Target identity:100%; Query identity:100%]
X-ray repair complementing defective repair in Chinese hamster cells 6 (Ku autoantigen, 70kDa), isoform CRA_a
Show all
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000723 [telomere maintenance]
GO:0000783 [nuclear telomere cap complex]
GO:0000784 [nuclear chromosome, telomeric region]
GO:0003677 [DNA binding]
GO:0003684 [damaged DNA binding]
GO:0003690 [double-stranded DNA binding]
GO:0003691 [double-stranded telomeric DNA binding]
GO:0004003 [ATP-dependent DNA helicase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005667 [transcription factor complex]
GO:0005829 [cytosol]
GO:0006266 [DNA ligation]
GO:0006281 [DNA repair]
GO:0006302 [double-strand break repair]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0006310 [DNA recombination]
GO:0006351 [transcription, DNA-templated]
GO:0008022 [protein C-terminus binding]
GO:0016020 [membrane]
GO:0016032 [viral process]
GO:0032481 [positive regulation of type I interferon production]
GO:0032508 [DNA duplex unwinding]
GO:0042162 [telomeric DNA binding]
GO:0043564 [Ku70:Ku80 complex]
GO:0044212 [transcription regulatory region DNA binding]
GO:0044822 [poly(A) RNA binding]
GO:0045087 [innate immune response]
GO:0045892 [negative regulation of transcription, DNA-templated]
GO:0045893 [positive regulation of transcription, DNA-templated]
GO:0045944 [positive regulation of transcription from RNA polymerase II promoter]
GO:0048660 [regulation of smooth muscle cell proliferation]
GO:0051575 [5'-deoxyribose-5-phosphate lyase activity]
GO:0070419 [nonhomologous end joining complex]
GO:0071480 [cellular response to gamma radiation]
GO:0071481 [cellular response to X-ray]
GO:0075713 [establishment of integrated proviral latency]
Show all
 609 aa
69.8 kDa 		No 0
XRCC6-201
ENSP00000403679
ENST00000428575
B1AHC9 [Direct mapping]
X-ray repair cross-complementing protein 6
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000723 [telomere maintenance]
GO:0003677 [DNA binding]
GO:0003684 [damaged DNA binding]
GO:0004003 [ATP-dependent DNA helicase activity]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0032508 [DNA duplex unwinding]
GO:0042162 [telomeric DNA binding]
GO:0043564 [Ku70:Ku80 complex]
Show all
 559 aa
64.3 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.