ADH1B - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

ADH1B

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA047814

Antibody HPA060902

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA047814

HPA060902

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

ADH1A - ENSG00000187758 [100%]
ADH1C - ENSG00000248144 [100%]

ADH1A - ENSG00000187758 [100%]
ADH1C - ENSG00000248144 [85%]

Released in version

References

VALIDATION SUMMARY

Antibody HPA047814

Antibody HPA060902

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA047814

Antibody HPA060902

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line SiHa shows localization to plasma membrane & cytosol.

Antibody dilution

1:24

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA047814

Antibody HPA060902

Standard validation

Supported

Figure description

Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.

Immunohistochemical staining of human liver shows moderate cytoplasmic and nuclear positivity in hepatocytes.

Expression

RNA: detected in 35 tissues
Protein: detected in 42 cell types

RNA: detected in 35 tissues
Protein: detected in 3 cell types

Retrieval

HIER pH6

Antibody dilution

1:700

1:25

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA047814

Antibody HPA060902

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

39.9, 39.8, 35.4

39.9, 39.8, 35.4, 19.8, 17.5

Antibody dilution

1:120

1:80

PROTEIN ARRAY

Antibody HPA047814

Antibody HPA060902

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:1000

1:500

ANTIGEN INFORMATION

Antibody HPA047814

Antibody HPA060902

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

EVIGRLDTMMASLLCCHEACGTSVIVG

IPQCGKCRICKNPESNYCLKNDVSNPQGTLQDGTSRFTCRRKPIHHF

Matching transcripts

ADH1B-001 - ENSP00000306606 [100%]
ADH1B-004 - ENSP00000425998 [100%]
ADH1B-201 - ENSP00000486614 [100%]

ADH1B-001 - ENSP00000306606 [85%]
ADH1B-004 - ENSP00000425998 [85%]
ADH1B-201 - ENSP00000486614 [85%]

Other gene match

ADH1A - ENSG00000187758 [100%]
ADH1C - ENSG00000248144 [100%]

ADH1A - ENSG00000187758 [100%]
ADH1C - ENSG00000248144 [85%]

ANTIGEN VIEW

ADH1B-001	ADH1B-004	ADH1B-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.