TCF4 - Antibodies - The Human Protein Atlas

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TCF4

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA025958

Antibody CAB020722

Provider

Atlas Antibodies
Sigma-Aldrich

SDIX

Product name

HPA025958

3036.00.02

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA025958

Antibody CAB020722

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA025958

Antibody CAB020722

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.

Antibody dilution

1:15

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA025958

Antibody CAB020722

Standard validation

Approved

Figure description

Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.

Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Expression

RNA: detected in 37 tissues
Protein: detected in 63 cell types

RNA: detected in 37 tissues
Protein: detected in 68 cell types

Retrieval

HIER pH6

Antibody dilution

1:15

1:60

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA025958

Antibody CAB020722

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Target mass (kDa)

83.4, 72.4, 71.8, 71.3, 71.2, 69.1, 68.6, 68.5, 67, 65.3, 64.8, 64.6, 64.1, 62.1, 57.4, 54.6, 54.2, 54.1, 48.1

83.4, 72.4, 71.8, 71.3, 71.2, 69.1, 68.6, 68.5, 67, 65.3, 64.8, 64.6, 64.1, 62.1, 57.4, 54.6, 54.2, 54.1, 48.1, 32.8, 30.8, 29.7, 25, 24.7, 23.8, 23.6, 22.3, 21.9, 21.5, 19.2, 19.1, 18.9, 18, 17.7, 17.6, 17, 16.8, 16.7, 15.7, 14.8, 14.7, 14.5, 13.9, 12.8, 9, 7.1, 6.1

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA025958

Antibody CAB020722

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA025958

Antibody CAB020722

Antigen

Recombinant protein fragment

Genetic immunization

Length (aa)

107

Antigen sequence

HGIIGPSHNGAMGGLGSGYGTGLLSANRHSLMVGTHREDGVALRGSHSLL
PNQVPVPQLPVQSATSPDLNPPQDPYRGMPPGLQGQSVSSGSSEIKSDDE
GDENLQD

Matching transcripts

TCF4-001 - ENSP00000346440 [100%]
TCF4-002 - ENSP00000348374 [100%]
TCF4-003 - ENSP00000440731 [100%]
TCF4-004 - ENSP00000381382 [100%]
TCF4-005 - ENSP00000455346 [100%]
TCF4-006 - ENSP00000457649 [100%]
TCF4-007 - ENSP00000445202 [100%]
TCF4-008 - ENSP00000455135 [100%]
TCF4-009 - ENSP00000439656 [100%]
TCF4-010 - ENSP00000455179 [100%]
TCF4-011 - ENSP00000441562 [100%]
TCF4-012 - ENSP00000454647 [100%]
TCF4-013 - ENSP00000439827 [100%]
TCF4-015 - ENSP00000409447 [100%]
TCF4-016 - ENSP00000457765 [100%]
TCF4-019 - ENSP00000457263 [100%]
TCF4-041 - ENSP00000455418 [100%]
TCF4-042 - ENSP00000455261 [100%]
TCF4-044 - ENSP00000455763 [100%]
TCF4-045 - ENSP00000456125 [100%]
TCF4-046 - ENSP00000454366 [100%]
TCF4-049 - ENSP00000486670 [100%]
TCF4-060 - ENSP00000486072 [100%]
TCF4-074 - ENSP00000455984 [100%]
TCF4-201 - ENSP00000478549 [100%]

Other gene match

ANTIGEN VIEW

TCF4-001	TCF4-002	TCF4-003	TCF4-004	TCF4-005	TCF4-006	TCF4-007	TCF4-008	TCF4-009	TCF4-010	TCF4-011	TCF4-012	TCF4-013	TCF4-015	TCF4-016	TCF4-019	TCF4-041	TCF4-042	TCF4-044	TCF4-045	TCF4-046	TCF4-049	TCF4-060	TCF4-074	TCF4-201

RELEVANT PUBLICATIONS

Antibody HPA025958

Antibody CAB020722

Sox4 functions as a positive regulator of β-catenin signaling through upregulation of TCF4 during morular differentiation of endometrial carcinomas
Saegusa M et al
Lab Invest 2012;92(4):511-21

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.