PDLIM7 - Antibodies - The Human Protein Atlas

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PDLIM7

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA018794

Antibody HPA048815

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA018794

HPA048815

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA018794

Antibody HPA048815

Immunocytochemistry

Immunohistochemistry

Approved

Western blot

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA018794

Antibody HPA048815

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA048815.

Validated

Antibody staining overlaps with antibody HPA018794.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to actin filaments & focal adhesion sites.

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus, actin filaments & focal adhesion sites.

Antibody dilution

1:50

1:51

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA018794

Antibody HPA048815

Standard validation

Approved

Figure description

Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.

Expression

RNA: detected in 37 tissues
Protein: detected in 18 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA018794

Antibody HPA048815

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

49.8, 46.5

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA018794

Antibody HPA048815

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA018794

Antibody HPA048815

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

HLTGTEFMQDPDEEHLKKSSQVPRTEAPAPASSTPQEPWPGPTAPSPTSR
PPWAVDPAFAERYAPDKTSTVLTR

GKTPVCHQCHKVIRGRYLVALGHAYHPEEFVCSQCGKVLEEGGFFEEKGA
IFCPPCYDVRYAPSCAKCKKKITGEIMHALKMTWH

Matching transcripts

PDLIM7-001 - ENSP00000348099 [100%]
PDLIM7-003 - ENSP00000352964 [100%]

Other gene match

ANTIGEN VIEW

PDLIM7-001	PDLIM7-003

RELEVANT PUBLICATIONS

Antibody HPA018794

Antibody HPA048815

The interactome of LIM domain proteins: the contributions of LIM domain proteins to heart failure and heart development
Li A et al
Proteomics 2012;12(2):203-25

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.