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DYNC1H1

ANTIBODY INFORMATION
 
 

Antibody HPA003742

  

Antibody CAB010443

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA003742   sc-9115  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 2   3  

References

 1    

VALIDATION SUMMARY
 
 

Antibody HPA003742

  

Antibody CAB010443

  
Immunocytochemistry

  Uncertain  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003742

  

Antibody CAB010443

  

Standard validation

   Uncertain  

   

Figure description

   Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & centrosome.  

Antibody dilution

   1:50  

Literature conformity

   The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003742

  

Antibody CAB010443

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in basal cells of the seminiferus ducts.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in spermatogonia.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 60 cell types		   RNA: detected in 37 tissues
Protein: detected in 64 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:200  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003742

  

Antibody CAB010443

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

   

Figure description

    

Target mass (kDa)

 532.4   532.4, 20.9  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA003742

  

Antibody CAB010443

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA003742

  

Antibody CAB010443

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 142    

Antigen sequence

 
RSLETCMYDHKTFSEILNRVQKAVDDLNLHSYSNLPIWVNKLDMEIERIL
GVRLQAGLRAWTQVLLGQAEDKAEVDMDTDAPQVSHKPGGEPKIKNVVHE
LRITNQVIYLNPPIEECRYKLYQEMFAWKMVVLSLPRIQSQR
  

											 
																				

																		

										
											
Matching transcripts

												
																					DYNC1H1-001 - ENSP00000348965 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
DYNC1H1-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA003742

												
										 
																				
											
											
											
Antibody CAB010443

												
										 
																		

																	

										
											
1.

												
																						Kinetochore motors drive congression of peripheral polar chromosomes by overcoming random arm-ejection forces

	Barisic M et al

	Nat Cell Biol 2014;16(12):1249-56

			Application: ICC-IF, WB				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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