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MYH6

ANTIBODY INFORMATION
 
 

Antibody HPA001239

  

Antibody HPA001349

  

Antibody CAB075723

  

Antibody CAB075724

  

Antibody CAB075725

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA001239   HPA001349   AMAb90947   AMAb90948   AMAb90950  

Host species

 Rabbit   Rabbit   Mouse   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G   Protein A/G  

Other gene match

 MYH7 - ENSG00000092054 [100%]
   MYH7 - ENSG00000092054 [87%]
MYH1 - ENSG00000109061 [81%]
MYH2 - ENSG00000125414 [82%]
MYH8 - ENSG00000133020 [80%]
MYH4 - ENSG00000264424 [80%]
        

Released in version

 1   1   14   14   14  

References

          

VALIDATION SUMMARY
 
 

Antibody HPA001239

  

Antibody HPA001349

  

Antibody CAB075723

  

Antibody CAB075724

  

Antibody CAB075725

  
Immunohistochemistry

Supported   Supported  

Validated
  

Validated
   Supported  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported        

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001239

  

Antibody HPA001349

  

Antibody CAB075723

  

Antibody CAB075724

  

Antibody CAB075725

  

Formal validation: Orthogonal

    
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

   
 

 

  
 

 

    

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Standard validation

 Supported   Supported   Supported   Supported   Supported  

         

Figure description

 Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in a subset of myocytes.   Immunohistochemical staining of human heart muscle shows cytoplasmic positivity in myocytes.   Immunohistochemical staining of human heart muscle shows cytoplasmic positivity in myocytes.   Immunohistochemical staining of human heart muscle shows cytoplasmic positivity in myocytes.  

Expression

 RNA: detected in 5 tissues
Protein: detected in 31 cell types		   RNA: detected in 5 tissues
Protein: detected in 5 cell types		   RNA: detected in 5 tissues
Protein: detected in 2 cell types		   RNA: detected in 5 tissues
Protein: detected in 2 cell types		   RNA: detected in 5 tissues
Protein: detected in 3 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:250   1:5000   1:5000   1:10000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001239

  

Antibody HPA001349

  

Antibody CAB075723

  

Antibody CAB075724

  

Antibody CAB075725

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

         

Figure description

          

Target mass (kDa)

 223.7, 223.1   223.7, 223.1, 223, 222.8   223.7   223.7   223.7  

Antibody dilution

 1:500   1:500   1:500   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001239

  

Antibody HPA001349

  

Antibody CAB075723

  

Antibody CAB075724

  

Antibody CAB075725

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		        

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.        

Antibody dilution

 1:2000   1:1000        

ANTIGEN INFORMATION
 
 

Antibody HPA001239

  

Antibody HPA001349

  

Antibody CAB075723

  

Antibody CAB075724

  

Antibody CAB075725

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Recombinant protein   Recombinant protein  

Length (aa)

 145   143        

Antigen sequence

 
ALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIK
AKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQ
LDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQS
  
QVEEDKVNSLSKSKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLK
LTQESIMDLENDKLQLEEKLKKKEFDINQQNSKIEDEQVLALQLQKKLKE
NQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEA
  

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					MYH6-001 - ENSP00000348634 [90%] 
MYH6-201 - ENSP00000386041 [90%] 

											 
																						MYH6-001 - ENSP00000348634 [100%] 
MYH6-201 - ENSP00000386041 [100%] 

											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					MYH7 - ENSG00000092054 [100%]

											 
																						MYH7 - ENSG00000092054 [87%]
MYH1 - ENSG00000109061 [81%]
MYH2 - ENSG00000125414 [82%]
MYH8 - ENSG00000133020 [80%]
MYH4 - ENSG00000264424 [80%]

											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
MYH6-001
MYH6-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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