MYO1C - Antibodies - The Human Protein Atlas

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MYO1C

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA001768

Antibody HPA005924

Antibody CAB037023

Provider

Atlas Antibodies
Sigma-Aldrich

SIGMA

Product name

HPA001768

HPA005924

M3567

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA001768

Antibody HPA005924

Antibody CAB037023

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA001768

Antibody HPA005924

Antibody CAB037023

Formal validation: Tagged protein

Validated

Antibody staining overlaps with GFP tagged protein

Figure description

Immunofluorescent staining of transgenic HeLa cells show antibody staining in plasma membrane and GFP expression in plasma membrane.

Antibody dilution

1:23

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & plasma membrane.

Antibody dilution

1:23

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA001768

Antibody HPA005924

Antibody CAB037023

Standard validation

Supported

Figure description

Immunohistochemical staining of human lung shows cytoplasmic and membranous positivity in pneumocytes.

Expression

RNA: detected in 37 tissues
Protein: detected in 72 cell types

RNA: detected in 37 tissues
Protein: detected in 73 cell types

Retrieval

HIER pH6

Antibody dilution

1:75

1:350

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA001768

Antibody HPA005924

Antibody CAB037023

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

121.7, 119.6, 119, 117.9

121.7, 119.6, 119, 117.9, 30.7, 19.5, 19.2, 18.7, 15.9, 15.4

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA001768

Antibody HPA005924

Antibody CAB037023

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA001768

Antibody HPA005924

Antibody CAB037023

Antigen

Recombinant protein fragment

Synthetic peptide

Length (aa)

148

Antigen sequence

GEEETLRRLGLERNPQSYLYLVKGQCAKVSSINDKSDWKVVRKALTVIDF
TEDEVEDLLSIVASVLHLGNIHFAANEESNAQVTTENQLKYLTRLLSVEG
STLREALTHRKIIAKGEELLSPLNLEQAAYARDALAKAVYSRTFTWLV

GEEETLRRLGLERNPQSYLYLVKGQCAKVSSINDKSDWKVVRKALTVIDF
TEDEVEDLLSIVASVLHLGNIHFAANEESNAQVTTENQLKYLTRLLSVEG
STLREALTHRKIIAKGEELLSPLNLEQAAYARDALAKAVYSRTFTWLV

Matching transcripts

MYO1C-001 - ENSP00000352834 [100%]
MYO1C-002 - ENSP00000459174 [100%]
MYO1C-003 - ENSP00000437685 [100%]
MYO1C-010 - ENSP00000412197 [100%]
MYO1C-201 - ENSP00000354283 [100%]