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Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA020100 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA020099 across 74 cell types.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in lymphoid tissue.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
Expression
RNA: detected in 35 tissues Protein: detected in 66 cell types
RNA: detected in 35 tissues Protein: detected in 46 cell types
RNA: detected in 35 tissues Protein: detected in 70 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:40
1:100
1:100
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA020099
Antibody HPA020100
Antibody CAB004280
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
147.9, 146.1
147.9, 146.1
147.9, 146.1, 21, 18.2
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA020099
Antibody HPA020100
Antibody CAB004280
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
