MBP - Antibodies - The Human Protein Atlas

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MBP

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA049222

Antibody HPA064368

Antibody CAB002300

Provider

Atlas Antibodies
Sigma-Aldrich

Novocastra

Product name

HPA049222

HPA064368

NCL-MBP

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA049222

Antibody HPA064368

Antibody CAB002300

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA049222

Antibody HPA064368

Antibody CAB002300

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.

Antibody dilution

1:76

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA049222

Antibody HPA064368

Antibody CAB002300

Standard validation

Supported

Figure description

Immunohistochemical staining of human cerebral cortex shows strong positivity in subsets of glial cells.

Immunohistochemical staining of human cerebral cortex shows distinct positivity in subsets of glial cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 5 cell types

RNA: detected in 37 tissues
Protein: detected in 8 cell types

Retrieval

HIER pH6

Antibody dilution

1:20000

1:150

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

Standard validation

Supported

Figure description

Staining of white matter structures and axon-like structures throughout the brain.

WESTERN BLOT

Antibody HPA049222

Antibody HPA064368

Antibody CAB002300

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

33.1, 21.5, 20.2, 19.7, 18.6, 17.3, 17.1, 16.2, 11.1

33.1, 27.7, 21.5, 17.4

33.1, 27.7, 25.7, 21.5, 20.2, 19.7, 19.4, 18.9, 18.6, 17.4, 17.3, 17.1, 16.2, 11.9, 11.1, 9.5, 7.9, 7.6, 4.5

Antibody dilution

1:1000

1:250

1:500

PROTEIN ARRAY

Antibody HPA049222

Antibody HPA064368

Antibody CAB002300

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:7950

1:3000

ANTIGEN INFORMATION

Antibody HPA049222

Antibody HPA064368

Antibody CAB002300

Antigen

Recombinant protein fragment

Not known

Length (aa)

Antigen sequence

DENPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQRPGFGYGGRA
SDYKSAHKGFKGVDAQGTLSKIFKLGGRDSRSGSPM

ASTNSETNRGESEKKRNLGELSRTTSEDNEVFGEADANQNNGTSSQDTAV
TDSKRTADPKNAWQDAHPADPGSRPHLIRLFSRDAP

Matching transcripts

MBP-002 - ENSP00000372025 [100%]
MBP-006 - ENSP00000380964 [100%]
MBP-028 - ENSP00000435641 [100%]
MBP-049 - ENSP00000462223 [100%]
MBP-201 - ENSP00000348273 [100%]
MBP-019 - ENSP00000380973 [90%]
MBP-001 - ENSP00000352667 [87%]
MBP-007 - ENSP00000380963 [87%]
MBP-016 - ENSP00000405882 [87%]
MBP-037 - ENSP00000463403 [87%]

MBP-004 - ENSP00000380961 [100%]
MBP-005 - ENSP00000380958 [100%]
MBP-035 - ENSP00000462374 [100%]
MBP-048 - ENSP00000463780 [100%]
MBP-049 - ENSP00000462223 [100%]
MBP-201 - ENSP00000348273 [100%]

Other gene match

ANTIGEN VIEW

MBP-001	MBP-002	MBP-004	MBP-005	MBP-006	MBP-007	MBP-016	MBP-019	MBP-028	MBP-035	MBP-037	MBP-048	MBP-049	MBP-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.