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ZNF335

ANTIBODY INFORMATION
 
 

Antibody HPA063999

  

Antibody HPA071230

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA063999   HPA071230  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA063999

  

Antibody HPA071230

  
Immunocytochemistry


Validated
  

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA063999

  

Antibody HPA071230

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA071230.		  
Validated

Antibody staining overlaps with antibody HPA063999.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.  

Antibody dilution

 1:127   1:51  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

WESTERN BLOT
 
 

Antibody HPA063999

  

Antibody HPA071230

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 144.9   144.9  

Antibody dilution

 1:420   1:170  

PROTEIN ARRAY
 
 

Antibody HPA063999

  

Antibody HPA071230

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:5100   1:2050  

ANTIGEN INFORMATION
 
 

Antibody HPA063999

  

Antibody HPA071230

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 79   85  

Antigen sequence

 
RNGHLKFHIQRLHSPDGRKSGTPTARAPTQTPTQTIILNSDDETLATLHT
ALQSSHGVLGPERLQQALSQEHIIVAQEQ
  
VTNQEEAAYIQEITTADGQTVQHLVTSDNQVQYIISQDGVQHLLPQEYVV
VPEGHHIQVQEGQITHIQYEQGAPFLQESQIQYVP
 

Matching transcripts

 ZNF335-001 - ENSP00000325326 [100%]
  ZNF335-001 - ENSP00000325326 [100%]
 

Other gene match

    
ANTIGEN VIEW
ZNF335-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.