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ANTIBODY INFORMATION
 
 

Antibody HPA046710

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA046710  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 10  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA046710

  
Immunocytochemistry


Validated
  
Immunohistochemistry

Approved  
Western blot


Validated
  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA046710

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		  

 

Relative fluorescence intensity (RFI)

  

Figure description

 Cytoplasmic region of segmented cells in 10x-images  

Antibody dilution

 1:200  
 

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol.  

Antibody dilution

 1:200  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA046710

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal and glial cells.  

Expression

 RNA: detected in 32 tissues
Protein: detected in 51 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:12000  

Literature conformity

 Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA046710

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		  

Target mass (kDa)

 140.5  

Loading control

 Total protein image  

Antibody dilution

 1:1140  
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 140.5  

Antibody dilution

 1:2570  

PROTEIN ARRAY
 
 

Antibody HPA046710

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:20550  

ANTIGEN INFORMATION
 
 

Antibody HPA046710

  

Antigen

 Recombinant protein fragment  

Length (aa)

 88  

Antigen sequence

 
AVALASDRCSIHLQLQGLVDPARELGKLQAKRVEAQRQAQRLRERRAASG
YPVKVPLEVQEADEAKLQQTEAELRKVDEAIALFQKML
 

Matching transcripts

 VARS-001 - ENSP00000364815 [100%]
 

Other gene match

  
ANTIGEN VIEW
VARS-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.