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KRT25

ANTIBODY INFORMATION
 
 

Antibody HPA053977

  

Antibody HPA058943

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA053977   HPA058943  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA053977

  

Antibody HPA058943

  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA053977

  

Antibody HPA058943

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA058943 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA053977 across 46 cell lines.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human hair follicles shows strong cytoplasmic positivity in cells in internal root sheath.   Immunohistochemical staining of human hair follicles shows strong cytoplasmic positivity in cells in internal root sheath.  

Expression

 RNA: detected in 2 tissues
Protein: detected in 0 cell types		   RNA: detected in 2 tissues
Protein: detected in 0 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1400   1:80  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA053977

  

Antibody HPA058943

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 49.3   49.3  

Antibody dilution

 1:580   1:80  

PROTEIN ARRAY
 
 

Antibody HPA053977

  

Antibody HPA058943

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:4600   1:650  

ANTIGEN INFORMATION
 
 

Antibody HPA053977

  

Antibody HPA058943

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 69   37  

Antigen sequence

 
TYCLLIGGDDGACKSGGYKSKDYGSGNVGSQVKDPAKAIVVKKVLEEVDQ
RSKILTTRLHSLEEKSQSN
  
RRSCPRPTTGSLRLYGGGTSFGTGNSCGISGIGSGFS
 

Matching transcripts

 KRT25-001 - ENSP00000310573 [100%]
  KRT25-001 - ENSP00000310573 [100%]
 

Other gene match

    
ANTIGEN VIEW
KRT25-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.