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PRSS1

ANTIBODY INFORMATION
 
 

Antibody HPA062452

  

Antibody HPA063471

  

Antibody CAB025487

  

Antibody CAB025538

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   R&D Systems
   R&D Systems
  

Product name

 HPA062452   HPA063471   MAB3586   MAB3848  

Host species

 Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

 PRSS3 - ENSG00000010438 [96%]
PRSS2 - ENSG00000275896 [84%]
   PRSS3 - ENSG00000010438 [97%]
PRSS2 - ENSG00000275896 [100%]
      

Released in version

 13   13   5   6  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA062452

  

Antibody HPA063471

  

Antibody CAB025487

  

Antibody CAB025538

  
Immunocytochemistry

Approved        
Immunohistochemistry

Supported   Supported   Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA062452

  

Antibody HPA063471

  

Antibody CAB025487

  

Antibody CAB025538

  

Standard validation

 Approved        

       

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles.        

Antibody dilution

 1:7        

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.        

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA062452

  

Antibody HPA063471

  

Antibody CAB025487

  

Antibody CAB025538

  

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human pancreas shows weak positivity in exocrine cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.  

Expression

 RNA: detected in 20 tissues
Protein: detected in 21 cell types		   RNA: detected in 20 tissues
Protein: detected in 6 cell types		   RNA: detected in 20 tissues
Protein: detected in 13 cell types		   RNA: detected in 20 tissues
Protein: detected in 1 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:350   1:4000   1:300   1:1200  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA062452

  

Antibody HPA063471

  

Antibody CAB025487

  

Antibody CAB025538

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

       

Figure description

        

Target mass (kDa)

 32.5, 28.4, 28.2, 28.1, 28, 26.8, 26.7, 26.6, 26.5, 26.4, 25.9, 25.4, 19.4, 15.4   32.5, 28.4, 28.2, 28.1, 28, 26.8, 26.7, 26.6, 26.5, 26.4, 25.9, 19.4, 15.4   28.1, 26.6, 25.4, 15.4   28.1, 26.6, 25.4, 15.4  

Antibody dilution

 1:80   1:290   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA062452

  

Antibody HPA063471

  

Antibody CAB025487

  

Antibody CAB025538

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:500   1:3550      

ANTIGEN INFORMATION
 
 

Antibody HPA062452

  

Antibody HPA063471

  

Antibody CAB025487

  

Antibody CAB025538

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Recombinant protein  

Length (aa)

 25   30      

Antigen sequence

 
NDIMLIKLSSRAVINARVSTISLPT
  
RIQVRLGEHNIEVLEGNEQFINAAKIIRHP
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					PRSS1-001 - ENSP00000308720 [100%] 
PRSS1-003 - ENSP00000417854 [100%] 
PRSS1-004 - ENSP00000419912 [100%] 
PRSS1-201 - ENSP00000479959 [100%] 
PRSS1-202 - ENSP00000481361 [100%] 

											 
																						PRSS1-001 - ENSP00000308720 [100%] 
PRSS1-003 - ENSP00000417854 [100%] 
PRSS1-004 - ENSP00000419912 [100%] 
PRSS1-201 - ENSP00000479959 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					PRSS3 - ENSG00000010438 [96%]
PRSS2 - ENSG00000275896 [84%]

											 
																						PRSS3 - ENSG00000010438 [97%]
PRSS2 - ENSG00000275896 [100%]

											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PRSS1-001
PRSS1-003
PRSS1-004
PRSS1-201
PRSS1-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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