PRSS3 - Antibodies - The Human Protein Atlas

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PRSS3

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA062452

Antibody HPA063471

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA062452

HPA063471

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

PRSS1 - ENSG00000204983 [100%]
PRSS2 - ENSG00000275896 [84%]

PRSS1 - ENSG00000204983 [100%]
PRSS2 - ENSG00000275896 [100%]

Released in version

References

VALIDATION SUMMARY

Antibody HPA062452

Antibody HPA063471

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA062452

Antibody HPA063471

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles.

Antibody dilution

1:7

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA062452

Antibody HPA063471

Standard validation

Supported

Figure description

Immunohistochemical staining of human pancreas shows weak positivity in exocrine cells.

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Expression

RNA: detected in 21 tissues
Protein: detected in 21 cell types

RNA: detected in 21 tissues
Protein: detected in 6 cell types

Retrieval

HIER pH6

Antibody dilution

1:350

1:4000

Literature conformity

Partly consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA062452

Antibody HPA063471

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Target mass (kDa)

32.5, 28.4, 28.2, 28.1, 28, 26.8, 26.7, 26.6, 26.5, 26.4, 25.9, 25.4, 19.4, 15.4

32.5, 28.4, 28.2, 28.1, 28, 26.8, 26.7, 26.6, 26.5, 26.4, 25.9, 19.4, 15.4

Antibody dilution

1:80

1:290

PROTEIN ARRAY

Antibody HPA062452

Antibody HPA063471

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:3550

ANTIGEN INFORMATION

Antibody HPA062452

Antibody HPA063471

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

NDIMLIKLSSRAVINARVSTISLPT

RIQVRLGEHNIEVLEGNEQFINAAKIIRHP

Matching transcripts

PRSS3-002 - ENSP00000401249 [96%]
PRSS3-003 - ENSP00000354280 [96%]
PRSS3-004 - ENSP00000368715 [96%]
PRSS3-201 - ENSP00000340889 [96%]
PRSS3-202 - ENSP00000401828 [96%]

PRSS3-002 - ENSP00000401249 [97%]
PRSS3-003 - ENSP00000354280 [97%]
PRSS3-004 - ENSP00000368715 [97%]
PRSS3-201 - ENSP00000340889 [97%]
PRSS3-202 - ENSP00000401828 [97%]

Other gene match

PRSS1 - ENSG00000204983 [100%]
PRSS2 - ENSG00000275896 [84%]

PRSS1 - ENSG00000204983 [100%]
PRSS2 - ENSG00000275896 [100%]

ANTIGEN VIEW

PRSS3-002	PRSS3-003	PRSS3-004	PRSS3-201	PRSS3-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.