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LGR4

ANTIBODY INFORMATION
 
 

Antibody HPA030267

  

Antibody HPA054350

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA030267   HPA054350  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   16  

References

 2    

VALIDATION SUMMARY
 
 

Antibody HPA030267

  

Antibody HPA054350

  
Immunocytochemistry

  Uncertain  
Immunohistochemistry

Uncertain    
Western blot

Supported   Uncertain  
Protein array

Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA030267

  

Antibody HPA054350

  

Standard validation

   Uncertain  

   

Figure description

   Immunofluorescent staining of human cell line HeLa shows localization to microtubule organizing center.  

Antibody dilution

   1:36  

Literature conformity

   The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA030267

  

Antibody HPA054350

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human breast shows strong positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 69 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:50    

Literature conformity

 Partly consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA030267

  

Antibody HPA054350

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 104.5, 101.7   104.5, 101.7  

Antibody dilution

 1:250   1:180  

PROTEIN ARRAY
 
 

Antibody HPA030267

  

Antibody HPA054350

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:30000   1:1450  

ANTIGEN INFORMATION
 
 

Antibody HPA030267

  

Antibody HPA054350

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 75   67  

Antigen sequence

 
KSHSCPALAVASCQRPEGYWSDCGTQSAHSDYADEEDSFVSDSSDQVQAC
GRACFYQSRGFPLVRYAYNLPRVKD
  
KEDWKLLKRRVTKKSGSVSVSISSQGGCLEQDFYYDCGMYSHLQGNLTVC
DCCESFLLTKPVSCKHL
 

Matching transcripts

 LGR4-001 - ENSP00000368516 [100%]
LGR4-002 - ENSP00000374508 [100%]
  LGR4-001 - ENSP00000368516 [100%]
LGR4-002 - ENSP00000374508 [100%]
 

Other gene match

    
ANTIGEN VIEW
LGR4-001
LGR4-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA030267

  

Antibody HPA054350

  

1.

 LGR4 and LGR6 are differentially expressed and of putative tumor biological significance in gastric carcinoma
Steffen JS et al
Virchows Arch 2012;461(4):355-65
    

2.

 LGR4 and LGR5 Regulate Hair Cell Differentiation in the Sensory Epithelium of the Developing Mouse Cochlea
Żak M et al
Front Cell Neurosci 2016;10:186
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.