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DENND1B

ANTIBODY INFORMATION
 
 

Antibody HPA042586

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042586  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 11  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA042586

  
Immunocytochemistry

Supported  
Immunohistochemistry

Uncertain  
Western blot

Supported  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA042586

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.  

Antibody dilution

 1:200  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042586

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:450  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042586

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 86.6, 45.8  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA042586

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA042586

  

Antigen

 Recombinant protein fragment  

Length (aa)

 81  

Antigen sequence

 
AFLCGGSGDQAEWNLGQDDSALHGKHLPPSPRKRVSSSGLTDSLFILKEE
NSNKHLGADNVSDPTSGLDFQLTSPEVSQTD
 

Matching transcripts

 DENND1B-001 - ENSP00000375839 [100%]
DENND1B-201 - ENSP00000479816 [100%]
 

Other gene match

  
ANTIGEN VIEW
DENND1B-001
DENND1B-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.