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LTB4R2

ANTIBODY INFORMATION
 
 

Antibody HPA029680

  

Antibody HPA072921

  

Antibody CAB022689

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA029680   HPA072921   AB9547  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 6   16   5  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA029680

  

Antibody HPA072921

  

Antibody CAB022689

  
Immunocytochemistry

  Supported    
Immunohistochemistry

Uncertain     Uncertain  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA029680

  

Antibody HPA072921

  

Antibody CAB022689

  

Standard validation

   Supported    

     

Figure description

   Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & plasma membrane.    

Antibody dilution

   1:10    

Literature conformity

   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA029680

  

Antibody HPA072921

  

Antibody CAB022689

  

Standard validation

 Uncertain     Uncertain  

     

Figure description

 Immunohistochemical staining of human fallopian tube shows distinct positivity in ciliated cells.     Immunohistochemical staining of human vagina shows distinct cytoplasmic positivity in superficial squamous epithelial cells.  

Expression

 RNA: detected in 31 tissues
Protein: detected in 37 cell types		     RNA: detected in 31 tissues
Protein: detected in 53 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:80     1:250  

Literature conformity

 Partly consistent with gene/protein characterization data.     Partly consistent with gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.     Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA029680

  

Antibody HPA072921

  

Antibody CAB022689

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 41.5   41.5, 37.9   41.5, 37.9, 33.8, 17.2  

Antibody dilution

 1:250   1:80   1:500  

PROTEIN ARRAY
 
 

Antibody HPA029680

  

Antibody HPA072921

  

Antibody CAB022689

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:550    

ANTIGEN INFORMATION
 
 

Antibody HPA029680

  

Antibody HPA072921

  

Antibody CAB022689

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 51   39    

Antigen sequence

 
MAPSHRASQVGFCPTPERPLWRLPPTCRPRRMSVCYRPPGNETLLSWKTS
R
  
SREGTMELRTTPQLKVVGQGRGNGDPGGGMEKDGPEWDL
  

											 
																				

																		

										
											
Matching transcripts

												
																					LTB4R2-001 - ENSP00000432146 [100%] 

											 
																						LTB4R2-001 - ENSP00000432146 [100%] 
LTB4R2-002 - ENSP00000433290 [100%] 
LTB4R2-201 - ENSP00000445772 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
LTB4R2-001
LTB4R2-002
LTB4R2-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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