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HBD

ANTIBODY INFORMATION
 
 

Antibody HPA043234

  

Antibody CAB037087

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SIGMA
  

Product name

 HPA043234   H4890  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

 HBG2 - ENSG00000196565 [92%]
HBE1 - ENSG00000213931 [92%]
HBG1 - ENSG00000213934 [92%]
HBB - ENSG00000244734 [100%]
    

Released in version

 10   7  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA043234

  

Antibody CAB037087

  
Immunocytochemistry

Approved    
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA043234

  

Antibody CAB037087

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to vesicles.    

Antibody dilution

 1:4    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043234

  

Antibody CAB037087

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human placenta shows erythrocyte positivity in vessels.   Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in subsets of bone marrow poietic cells.  

Expression

 RNA: detected in 5 tissues
Protein: detected in 6 cell types		   RNA: detected in 5 tissues
Protein: detected in 17 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:10   1:3000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA043234

  

Antibody CAB037087

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 16.2, 16.1, 16, 15.3   16.1, 15.4, 11.3, 9.8  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA043234

  

Antibody CAB037087

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA043234

  

Antibody CAB037087

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 24    

Antigen sequence

 
FATLSELHCDKLHVDPENFRLLGN
   

Matching transcripts

 HBD-001 - ENSP00000369654 [92%]
   

Other gene match

 HBG2 - ENSG00000196565 [92%]
HBE1 - ENSG00000213931 [92%]
HBG1 - ENSG00000213934 [92%]
HBB - ENSG00000244734 [100%]
    
ANTIGEN VIEW
HBD-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.