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Immunofluorescent staining of human cell line RT4 shows localization to centrosome.
Antibody dilution
1:9
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA048884
Standard validation
Approved
Figure description
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of non germinal and germinal center cells.
Expression
RNA: detected in 11 tissues Protein: detected in 35 cell types
Retrieval
HIER pH6
Antibody dilution
1:50
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA048884
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
25.4
Antibody dilution
1:90
PROTEIN ARRAY
Antibody HPA048884
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
