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AMACR

ANTIBODY INFORMATION
 
 

Antibody HPA019527

  

Antibody HPA020912

  

Antibody CAB001809

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
  

Product name

 HPA019527   HPA020912   M3616  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Supernatant  

Other gene match

      

Released in version

 7   7   1  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA019527

  

Antibody HPA020912

  

Antibody CAB001809

  
Immunocytochemistry

  Supported    
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  

Validated
  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA019527

  

Antibody HPA020912

  

Antibody CAB001809

  

Standard validation

   Supported    

     

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & vesicles.    

Antibody dilution

   1:100    

Literature conformity

   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA019527

  

Antibody HPA020912

  

Antibody CAB001809

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  
 

 

  
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 

  
 

    

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA020912 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA019527 across 46 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules.   Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in subsets of renal tubuli.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 26 cell types		   RNA: detected in 37 tissues
Protein: detected in 14 cell types		   RNA: detected in 37 tissues
Protein: detected in 3 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:5000   1:250   1:100  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA019527

  

Antibody HPA020912

  

Antibody CAB001809

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 43.9, 42.4, 40.7   43.9, 42.4, 40.7   43.9, 42.4, 40.7, 31.7, 30.8, 28.5, 22.2  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA019527

  

Antibody HPA020912

  

Antibody CAB001809

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA019527

  

Antibody HPA020912

  

Antibody CAB001809

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 75   84    

Antigen sequence

 
HNKERGSFITSEEQDVSPRPAPLLLNTPAIPSFKRDPFIGEHTEEILEEF
GFSREEIYQLNSDKIIESNKVKASL
  
APFYTTYRTADGEFMAVGAIEPQFYELLIKGLGLKSDELPNQMSMDDWPE
MKKKFADVFAKKTKAEWCQIFDGTDACVTPVLTF
   

Matching transcripts

 AMACR-001 - ENSP00000334424 [100%]
AMACR-003 - ENSP00000424351 [100%]
AMACR-008 - ENSP00000371517 [93%]
  AMACR-001 - ENSP00000334424 [99%]
AMACR-003 - ENSP00000424351 [99%]
AMACR-008 - ENSP00000371517 [99%]
   

Other gene match

      
ANTIGEN VIEW
AMACR-001
AMACR-003
AMACR-008
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.