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Immunohistochemical staining of human placenta shows cytoplasmic positivity in cytotrophoblasts.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in cytotrophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in a subset of trophoblastic cells.
Expression
RNA: detected in 33 tissues Protein: detected in 33 cell types
RNA: detected in 33 tissues Protein: detected in 24 cell types
RNA: detected in 33 tissues Protein: detected in 4 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:75
1:15
1:600
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA021324
Antibody HPA029915
Antibody HPA051038
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
88.2, 83.6, 80.1
88.2, 83.6, 80.1
88.2, 83.6, 80.1, 45.1, 40.5, 37
Antibody dilution
1:250
1:250
1:360
PROTEIN ARRAY
Antibody HPA021324
Antibody HPA029915
Antibody HPA051038
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
