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Immunofluorescent staining of human cell line HaCaT shows localization to cytosol.
Antibody dilution
1:45
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA002736
Antibody HPA028623
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human fallopian tube shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows strong positivity in cilated cells.
Expression
RNA: detected in 10 tissues Protein: detected in 37 cell types
RNA: detected in 10 tissues Protein: detected in 6 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:50
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA002736
Antibody HPA028623
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
149, 145, 78.2
149, 145
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA002736
Antibody HPA028623
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
