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Immunohistochemical staining of human urinary bladder shows positivity in plasma.
Immunohistochemical staining of human rectum shows positivity in plasma.
Immunohistochemical staining of human duodenum shows positivity in plasma.
Immunohistochemical staining of human colon shows distinct positivity in plasma.
Immunohistochemical staining of human colon shows distinct positivity in plasma.
Expression
RNA: detected in 25 tissues Protein: detected in 21 cell types
RNA: detected in 25 tissues Protein: detected in 18 cell types
RNA: detected in 25 tissues Protein: detected in 44 cell types
RNA: detected in 25 tissues Protein: detected in 49 cell types
RNA: detected in 25 tissues Protein: detected in 12 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:40
1:40
1:25
1:100
1:175
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA046356
Antibody HPA048287
Antibody HPA050103
Antibody CAB009811
Antibody CAB032603
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
192.8, 192.7, 187.7
192.8, 192.7, 187.7
192.8, 192.7, 187.7
192.8, 187.7
192.8, 187.7
Antibody dilution
1:250
1:230
1:130
1:500
1:500
PROTEIN ARRAY
Antibody HPA046356
Antibody HPA048287
Antibody HPA050103
Antibody CAB009811
Antibody CAB032603
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
