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Immunofluorescent staining of human cell line CACO-2 shows localization to mitochondria.
Antibody dilution
1:46
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA020106
Antibody HPA047679
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity.
Expression
RNA: detected in 34 tissues Protein: detected in 67 cell types
RNA: detected in 34 tissues Protein: detected in 33 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:125
1:75
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA020106
Antibody HPA047679
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
84.1, 78.9
84.1, 78.9
Antibody dilution
1:250
1:100
PROTEIN ARRAY
Antibody HPA020106
Antibody HPA047679
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:1800
ANTIGEN INFORMATION
Antibody HPA020106
Antibody HPA047679
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
103
126
Antigen sequence
PVELEVNLLTPGAVPPTVVSFGTVTTPADRLALIGGLEGFLSKMAEHRQQ
PDIRTLTLLAEVVESGSPAESLLLALLDEHQVEADLTFFNTLVRKKSKLG
DLE
