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Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Antibody dilution
1:50
1:100
1:400
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA022853
Antibody HPA023043
Antibody HPA023048
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human colon shows strong cytoplasmic positivity with a granular pattern in glandular cells.
Immunohistochemical staining of human colon shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human kidney shows strong cytopmasmic positivity with granular pattern in cells in tubules and cells in glomeruli.
Expression
RNA: detected in 2 tissues Protein: detected in 77 cell types
RNA: detected in 2 tissues Protein: detected in 81 cell types
RNA: detected in 2 tissues Protein: detected in 64 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:500
1:500
1:450
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA022853
Antibody HPA023043
Antibody HPA023048
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415953)
Target mass (kDa)
48.1, 21.3
48.1, 21.3, 19.5
48.1, 43.6, 32.1, 31.3
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA022853
Antibody HPA023043
Antibody HPA023048
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
