We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Antibody dilution
1:400
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA023048
Standard validation
Supported
Figure description
Immunohistochemical staining of human kidney shows strong cytopmasmic positivity with granular pattern in cells in tubules and cells in glomeruli.
Expression
RNA: detected in 36 tissues Protein: detected in 64 cell types
Retrieval
HIER pH6
Antibody dilution
1:450
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA023048
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415953)
Target mass (kDa)
48.1, 43.6, 32.1, 31.3
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA023048
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
