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ZNF285

ANTIBODY INFORMATION
 
 

Antibody HPA046100

  

Antibody HPA049212

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA046100   HPA049212  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA046100

  

Antibody HPA049212

  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Supported   Uncertain  
Protein array

Approved   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA046100

  

Antibody HPA049212

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 29 tissues
Protein: detected in 51 cell types		   RNA: detected in 29 tissues
Protein: detected in 65 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:40   1:650  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA046100

  

Antibody HPA049212

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 68.9, 68.1   68.9, 68.1  

Antibody dilution

 1:250   1:470  

PROTEIN ARRAY
 
 

Antibody HPA046100

  

Antibody HPA049212

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3800  

ANTIGEN INFORMATION
 
 

Antibody HPA046100

  

Antibody HPA049212

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 78   84  

Antigen sequence

 
TQVLTPESWRKANIMTEPQNSQGRYKGIYMEEKLYRRAQHDDSLSWTSCD
HHESQECKGEDPGRHPSCGKNLGMKSTV
  
LNLQAKGLSYLSQEVLHCWQIWKQRIRDLTVSQDYIVNLQEECSPHLEDV
SLSEEWAGISLQISENENYVVNAIIKNQDITAWQ
 

Matching transcripts

 ZNF285-001 - ENSP00000439431 [100%]
ZNF285-002 - ENSP00000483662 [100%]
ZNF285-005 - ENSP00000464788 [100%]
  ZNF285-001 - ENSP00000439431 [100%]
ZNF285-002 - ENSP00000483662 [100%]
ZNF285-005 - ENSP00000464788 [100%]
 

Other gene match

    
ANTIGEN VIEW
ZNF285-001
ZNF285-002
ZNF285-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.